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4r84
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4r84 is ON HOLD until Paper Publication Authors: Fisher, A.J., Chen, X., Li, Y., Huynh, N. Description: Crystal structure of Sialyltransferase from...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Sialyltransferase from Photobacterium damsela with CMP-3F(a)Neu5Ac bound== | |
| + | <StructureSection load='4r84' size='340' side='right'caption='[[4r84]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4r84]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Photobacterium_damselae Photobacterium damselae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R84 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R84 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CSF:CYTIDINE-5-MONOPHOSPHATE-3-FLUORO-N-ACETYL-NEURAMINIC+ACID'>CSF</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r84 OCA], [https://pdbe.org/4r84 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r84 RCSB], [https://www.ebi.ac.uk/pdbsum/4r84 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r84 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/O66375_9GAMM O66375_9GAMM] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Sialyltransferase structures fall into either GT-A or GT-B glycosyltransferase fold. Some sialyltransferases from the Photobacterium genus have been shown to contain an additional N-terminal immunoglobulin (Ig)-like domain. Photobacterium damselae alpha2-6-sialyltransferase has been used efficiently in enzymatic and chemoenzymatic synthesis of alpha2-6-linked sialosides. Here we report three crystal structures of this enzyme. Two structures with and without a donor substrate analog CMP-3F(a)Neu5Ac contain an immunoglobulin (Ig)-like domain and adopt the GT-B sialyltransferase fold. The binary structure reveals a non-productive pre-Michaelis complex, which are caused by crystal lattice contacts that prevent the large conformational changes. The third structure lacks the Ig-domain. Comparison of the three structures reveals small inherent flexibility between the two Rossmann-like domains of the GT-B fold. | ||
| - | + | Crystal structures of sialyltransferase from Photobacterium damselae.,Huynh N, Li Y, Yu H, Huang S, Lau K, Chen X, Fisher AJ FEBS Lett. 2014 Dec 20;588(24):4720-9. doi: 10.1016/j.febslet.2014.11.003. Epub, 2014 Nov 15. PMID:25451227<ref>PMID:25451227</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4r84" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Sialyltransferase 3D structures|Sialyltransferase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Photobacterium damselae]] | ||
| + | [[Category: Chen X]] | ||
| + | [[Category: Fisher AJ]] | ||
| + | [[Category: Huynh N]] | ||
| + | [[Category: Li Y]] | ||
Current revision
Crystal structure of Sialyltransferase from Photobacterium damsela with CMP-3F(a)Neu5Ac bound
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