1e8r

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[[Image:1e8r.gif|left|200px]]
 
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{{Structure
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==SOLUTION STRUCTURE OF TYPE X CBD==
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|PDB= 1e8r |SIZE=350|CAPTION= <scene name='initialview01'>1e8r</scene>
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<StructureSection load='1e8r' size='340' side='right'caption='[[1e8r]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1e8r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ct7 1ct7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E8R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E8R FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e8r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e8r OCA], [https://pdbe.org/1e8r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e8r RCSB], [https://www.ebi.ac.uk/pdbsum/1e8r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e8r ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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'''SOLUTION STRUCTURE OF TYPE X CBD'''
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[https://www.uniprot.org/uniprot/XYNA_CELJU XYNA_CELJU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Plant cell wall hydrolases generally have a modular structure consisting of a catalytic domain linked to one or more noncatalytic carbohydrate-binding modules (CBMs), whose common function is to attach the enzyme to the polymeric substrate. Xylanase A from Pseudomonas fluorescens subsp. cellulosa (Pf Xyn10A) consists of a family 10 catalytic domain, an N-terminal family IIa cellulose-binding module, and an internal family 10 cellulose-binding module. The structure of the 45-residue family 10 CBM has been determined in solution using NMR. It consists of two antiparallel beta-sheets, one with two strands and one with three, with a short alpha-helix across one face of the three-stranded sheet. There is a high density of aromatic residues on one side of the protein, including three aromatic residues (Tyr8, Trp22, and Trp24), which are exposed and form a flat surface on one face, in a classical polysaccharide-binding arrangement. The fold is closely similar to that of the oligonucleotide/oligosaccharide-binding (OB) fold, but appears to have arisen by convergent evolution, because there is no sequence similarity, and the presumed binding sites are on different faces.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e8/1e8r_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1E8R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. This structure supersedes the now removed PDB entry 1CT7. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E8R OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e8r ConSurf].
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Solution structure of the CBM10 cellulose binding module from Pseudomonas xylanase A., Raghothama S, Simpson PJ, Szabo L, Nagy T, Gilbert HJ, Williamson MP, Biochemistry. 2000 Feb 8;39(5):978-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10653641 10653641]
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<div style="clear:both"></div>
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[[Category: Endo-1,4-beta-xylanase]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas fluorescens]]
[[Category: Pseudomonas fluorescens]]
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[[Category: Single protein]]
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[[Category: Gilbert HJ]]
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[[Category: Gilbert, H J.]]
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[[Category: Raghothama S]]
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[[Category: Raghothama, S.]]
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[[Category: Simpson PJ]]
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[[Category: Simpson, P J.]]
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[[Category: Williamson MP]]
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[[Category: Williamson, M P.]]
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[[Category: antiparallel sheet]]
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[[Category: beta strand]]
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[[Category: hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:51:41 2008''
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Current revision

SOLUTION STRUCTURE OF TYPE X CBD

PDB ID 1e8r

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OCA

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