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2csm

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[[Image:2csm.gif|left|200px]]<br />
 
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<applet load="2csm" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2csm, resolution 2.8&Aring;" />
 
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'''TYR-BOUND T-STATE OF YEAST CHORISMATE MUTASE'''<br />
 
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==Overview==
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==TYR-BOUND T-STATE OF YEAST CHORISMATE MUTASE==
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The crystal structure of the tyrosine-bound T state of allosteric yeast, Saccharomyces cerevisiae chorismate mutase was solved by molecular, replacement at a resolution of 2.8 angstroms using a monomer of the, R-state structure as the search model. The allosteric inhibitor tyrosine, was found to bind in the T state at the same binding site as the, allosteric activator tryptophan binds in the R state, thus defining one, regulatory binding site for each monomer. Activation by tryptophan is, caused by the larger steric size of its side chain, thereby pushing apart, the allosteric domain of one monomer and helix H8 of the catalytic domain, of the other monomer. Inhibition is caused by polar contacts of tyrosine, with Arg-75 and Arg-76 of one monomer and with Gly-141, Ser-142, and, Thr-145 of ... [[http://ispc.weizmann.ac.il/pmbin/getpm?8622937 (full description)]]
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<StructureSection load='2csm' size='340' side='right'caption='[[2csm]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2csm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CSM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CSM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TYR:TYROSINE'>TYR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2csm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2csm OCA], [https://pdbe.org/2csm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2csm RCSB], [https://www.ebi.ac.uk/pdbsum/2csm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2csm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CHMU_YEAST CHMU_YEAST]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cs/2csm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2csm ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2CSM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with TYR as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Chorismate_mutase Chorismate mutase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.5 5.4.99.5]]. Structure known Active Sites: CAT and REG. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CSM OCA]].
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*[[3D structures of chorismate mutase|3D structures of chorismate mutase]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of the T state of allosteric yeast chorismate mutase and comparison with the R state., Strater N, Hakansson K, Schnappauf G, Braus G, Lipscomb WN, Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3330-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8622937 8622937]
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[[Category: Large Structures]]
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[[Category: Chorismate mutase]]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Hakansson K]]
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[[Category: Hakansson, K.]]
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[[Category: Lipscomb WN]]
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[[Category: Lipscomb, W.N.]]
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[[Category: Straeter N]]
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[[Category: Straeter, N.]]
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[[Category: TYR]]
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[[Category: allosteric protein]]
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[[Category: complex (isomerase/peptide)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 17:14:05 2007''
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Current revision

TYR-BOUND T-STATE OF YEAST CHORISMATE MUTASE

PDB ID 2csm

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