4q76

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of Nfs2 C384S mutant, the plastidial cysteine desulfurase from Arabidopsis thaliana

Structural highlights

4q76 is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.9Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CNIF1_ARATH Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L-selenocystine to produce L-alanine (PubMed:12033984, PubMed:16455656). Supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters (PubMed:15480755, PubMed:17372218). Required for the maturation of all plastidic Fe-S proteins and, thus, essential for plant growth (PubMed:17372218).^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

The chloroplastic Arabidopsis thaliana Nfs2 (AtNfs2) is a group II pyridoxal 5'-phosphate-dependent cysteine desulfurase that is involved in the initial steps of iron-sulfur cluster biogenesis. The group II cysteine desulfurases require the presence of sulfurtransferases such as SufE proteins for optimal activity. Compared with group I cysteine desulfurases, proteins of this group contains a smaller extended lobe harbouring the catalytic cysteine and have a beta-hairpin constraining the active site. Here, two crystal structures of AtNfs2 are reported: a wild-type form with the catalytic cysteine in a persulfide-intermediate state and a C384S variant mimicking the resting state of the enzyme. In both structures the well conserved Lys241 covalently binds pyridoxal 5'-phosphate, forming an internal aldimine. Based on available homologous bacterial complexes, a model of a complex between AtNfs2 and the SufE domain of its biological partner AtSufE1 is proposed, revealing the nature of the binding sites.

X-ray structures of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana.,Roret T, Pegeot H, Couturier J, Mulliert G, Rouhier N, Didierjean C Acta Crystallogr F Struct Biol Commun. 2014 Sep 1;70(Pt 9):1180-5. doi:, 10.1107/S2053230X14017026. Epub 2014 Aug 29. PMID:25195888^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Léon S, Touraine B, Briat JF, Lobréaux S. The AtNFS2 gene from Arabidopsis thaliana encodes a NifS-like plastidial cysteine desulphurase. Biochem J. 2002 Sep 1;366(Pt 2):557-64. PMID:12033984 doi:10.1042/BJ20020322
↑ Ye H, Garifullina GF, Abdel-Ghany SE, Zhang L, Pilon-Smits EA, Pilon M. The chloroplast NifS-like protein of Arabidopsis thaliana is required for iron-sulfur cluster formation in ferredoxin. Planta. 2005 Feb;220(4):602-8. PMID:15480755 doi:10.1007/s00425-004-1388-1
↑ Ye H, Abdel-Ghany SE, Anderson TD, Pilon-Smits EA, Pilon M. CpSufE activates the cysteine desulfurase CpNifS for chloroplastic Fe-S cluster formation. J Biol Chem. 2006 Mar 31;281(13):8958-69. Epub 2006 Feb 2. PMID:16455656 doi:http://dx.doi.org/10.1074/jbc.M512737200
↑ Van Hoewyk D, Abdel-Ghany SE, Cohu CM, Herbert SK, Kugrens P, Pilon M, Pilon-Smits EA. Chloroplast iron-sulfur cluster protein maturation requires the essential cysteine desulfurase CpNifS. Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5686-91. PMID:17372218 doi:10.1073/pnas.0700774104
↑ Roret T, Pegeot H, Couturier J, Mulliert G, Rouhier N, Didierjean C. X-ray structures of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana. Acta Crystallogr F Struct Biol Commun. 2014 Sep 1;70(Pt 9):1180-5. doi:, 10.1107/S2053230X14017026. Epub 2014 Aug 29. PMID:25195888 doi:http://dx.doi.org/10.1107/S2053230X14017026

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4q76"

Categories: Arabidopsis thaliana | Large Structures | Didierjean C | Roret T

@@ Line 1: / Line 1: @@
 ==Crystal structure of Nfs2 C384S mutant, the plastidial cysteine desulfurase from Arabidopsis thaliana==
-<StructureSection load='4q76' size='340' side='right' caption='[[4q76]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='4q76' size='340' side='right'caption='[[4q76]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4q76]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q76 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q76 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4q76]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q76 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q76 FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=LLP:2-LYSINE(3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHANE)'>LLP</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4q75|4q75]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LLP:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>LLP</scene></td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q76 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q76 RCSB], [http://www.ebi.ac.uk/pdbsum/4q76 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q76 OCA], [https://pdbe.org/4q76 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q76 RCSB], [https://www.ebi.ac.uk/pdbsum/4q76 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q76 ProSAT]</span></td></tr>
-<table>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CNIF1_ARATH CNIF1_ARATH] Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L-selenocystine to produce L-alanine (PubMed:12033984, PubMed:16455656). Supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters (PubMed:15480755, PubMed:17372218). Required for the maturation of all plastidic Fe-S proteins and, thus, essential for plant growth (PubMed:17372218).<ref>PMID:12033984</ref> <ref>PMID:15480755</ref> <ref>PMID:16455656</ref> <ref>PMID:17372218</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 15: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4q76" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Cysteine desulfurase 3D structures|Cysteine desulfurase 3D structures]]
+*[[Selenocysteine lyase|Selenocysteine lyase]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Didierjean, C.]]
+[[Category: Arabidopsis thaliana]]
-[[Category: Roret, T.]]
+[[Category: Large Structures]]
-[[Category: Atsufe1]]
+[[Category: Didierjean C]]
-[[Category: Chloroplast]]
+[[Category: Roret T]]
-[[Category: Cysteine desulfurase]]
-[[Category: Transferase]]

4q76

From Proteopedia

Current revision

Crystal structure of Nfs2 C384S mutant, the plastidial cysteine desulfurase from Arabidopsis thaliana

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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