4ocb

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==Z-DNA dodecamer d(CGCGCGCGCGCG)2 at 0.75 A resolution solved by P-SAD==
==Z-DNA dodecamer d(CGCGCGCGCGCG)2 at 0.75 A resolution solved by P-SAD==
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<StructureSection load='4ocb' size='340' side='right' caption='[[4ocb]], [[Resolution|resolution]] 0.75&Aring;' scene=''>
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<StructureSection load='4ocb' size='340' side='right'caption='[[4ocb]], [[Resolution|resolution]] 0.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4ocb]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OCB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OCB FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4ocb]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OCB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OCB FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3p4j|3p4j]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.75&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ocb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ocb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ocb RCSB], [http://www.ebi.ac.uk/pdbsum/4ocb PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ocb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ocb OCA], [https://pdbe.org/4ocb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ocb RCSB], [https://www.ebi.ac.uk/pdbsum/4ocb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ocb ProSAT]</span></td></tr>
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<table>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A large number of Z-DNA hexamer duplex structures and a few oligomers of different lengths are available, but here the first crystal structure of the d(CGCGCGCGCGCG)2 dodecameric duplex is presented. Two synchrotron data sets were collected; one was used to solve the structure by the single-wavelength anomalous dispersion (SAD) approach based on the anomalous signal of P atoms, the other set, extending to an ultrahigh resolution of 0.75 A, served to refine the atomic model to an R factor of 12.2% and an R(free) of 13.4%. The structure consists of parallel duplexes arranged into practically infinitely long helices packed in a hexagonal fashion, analogous to all other known structures of Z-DNA oligomers. However, the dodecamer molecule shows a high level of flexibility, especially of the backbone phosphate groups, with six out of 11 phosphates modeled in double orientations corresponding to the two previously observed Z-DNA conformations: Z(I), with the phosphate groups inclined towards the inside of the helix, and Z(II), with the phosphate groups rotated towards the outside of the helix.
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Phosphates in the Z-DNA dodecamer are flexible, but their P-SAD signal is sufficient for structure solution.,Luo Z, Dauter M, Dauter Z Acta Crystallogr D Biol Crystallogr. 2014 Jul;70(Pt 7):1790-800. doi:, 10.1107/S1399004714004684. Epub 2014 Jun 24. PMID:25004957<ref>PMID:25004957</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Dauter, M.]]
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[[Category: Large Structures]]
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[[Category: Dauter, Z.]]
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[[Category: Dauter M]]
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[[Category: Luo, Z.]]
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[[Category: Dauter Z]]
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[[Category: Dna]]
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[[Category: Luo Z]]
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[[Category: Z-dna dodecamer duplex]]
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Current revision

Z-DNA dodecamer d(CGCGCGCGCGCG)2 at 0.75 A resolution solved by P-SAD

PDB ID 4ocb

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