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3wz1
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Catalytic domain of beta-agarase from Microbulbifer thermotolerans JAMB-A94== | |
| + | <StructureSection load='3wz1' size='340' side='right'caption='[[3wz1]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3wz1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Microbulbifer_thermotolerans Microbulbifer thermotolerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WZ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WZ1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wz1 OCA], [https://pdbe.org/3wz1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wz1 RCSB], [https://www.ebi.ac.uk/pdbsum/3wz1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wz1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q6F4N4_9GAMM Q6F4N4_9GAMM] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A deep-sea bacterium, Microbulbifer thermotolerans JAMB-A94, has a beta-agarase (MtAgaA) belonging to the glycoside hydrolase family (GH) 16. The optimal temperature of this bacterium for growth is 43-49 degrees C, and MtAgaA is stable at 60 degrees C, which is one of the most thermostable enzymes among GH16 beta-agarases. Here, we determined the catalytic domain structure of MtAgaA. MtAgaA consists of a beta-jelly roll fold, as observed in other GH16 enzymes. The structure of MtAgaA was most similar to two beta-agarases from Zobellia galactanivorans, ZgAgaA, and ZgAgaB. Although the catalytic cleft structure of MtAgaA was similar to ZgAgaA and ZgAgaB, residues at subsite -4 of MtAgaA were not conserved between them. Also, an alpha-helix, designated as alpha4', was uniquely located near the catalytic cleft of MtAgaA. A comparison of the structures of the three enzymes suggested that multiple factors, including increased numbers of arginine and proline residues, could contribute to the thermostability of MtAgaA. | ||
| - | + | Crystal structure of the catalytic domain of a GH16 beta-agarase from a deep-sea bacterium, Microbulbifer thermotolerans JAMB-A94.,Takagi E, Hatada Y, Akita M, Ohta Y, Yokoi G, Miyazaki T, Nishikawa A, Tonozuka T Biosci Biotechnol Biochem. 2015 Apr;79(4):625-32. doi:, 10.1080/09168451.2014.988680. Epub 2014 Dec 6. PMID:25483365<ref>PMID:25483365</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 3wz1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Microbulbifer thermotolerans]] | ||
| + | [[Category: Akita M]] | ||
| + | [[Category: Hatada Y]] | ||
| + | [[Category: Miyazaki T]] | ||
| + | [[Category: Nishikawa A]] | ||
| + | [[Category: Ohta Y]] | ||
| + | [[Category: Takagi E]] | ||
| + | [[Category: Tonozuka T]] | ||
| + | [[Category: Yokoi G]] | ||
Current revision
Catalytic domain of beta-agarase from Microbulbifer thermotolerans JAMB-A94
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