1elb

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[[Image:1elb.gif|left|200px]]
 
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{{Structure
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==Analogous inhibitors of elastase do not always bind analogously==
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|PDB= 1elb |SIZE=350|CAPTION= <scene name='initialview01'>1elb</scene>, resolution 2.1&Aring;
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<StructureSection load='1elb' size='340' side='right'caption='[[1elb]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1elb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ELB FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0Z4:6-AMMONIO-N-(TRIFLUOROACETYL)-L-NORLEUCYL-N-[4-(1-METHYLETHYL)PHENYL]-L-LEUCINAMIDE'>0Z4</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1elb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1elb OCA], [https://pdbe.org/1elb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1elb RCSB], [https://www.ebi.ac.uk/pdbsum/1elb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1elb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CELA1_PIG CELA1_PIG] Acts upon elastin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/el/1elb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1elb ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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It has been assumed that the structure of a single inhibitor complex is sufficient to define the available subsites of an enzyme that has a unique binding site and a uniquely defined mode for ligand binding--the specificity for these subsites can thus be probed by kinetic experiments. Elastase is an enzyme for which these traditional assumptions, which underlie such structural and kinetic studies, do not hold. Three new crystal structures of elastase complexed to chemically similar inhibitors with similar binding affinities reveal a diversity of binding modes as well as two new subsites on elastase. The existence of multiple binding sites and different binding modes for such similar inhibitors indicates that researchers must proceed with caution when using kinetics to map out protein subsites.
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'''ANALOGOUS INHIBITORS OF ELASTASE DO NOT ALWAYS BIND ANALOGOUSLY'''
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Analogous inhibitors of elastase do not always bind analogously.,Mattos C, Rasmussen B, Ding X, Petsko GA, Ringe D Nat Struct Biol. 1994 Jan;1(1):55-8. PMID:7656008<ref>PMID:7656008</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1elb" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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It has been assumed that the structure of a single inhibitor complex is sufficient to define the available subsites of an enzyme that has a unique binding site and a uniquely defined mode for ligand binding--the specificity for these subsites can thus be probed by kinetic experiments. Elastase is an enzyme for which these traditional assumptions, which underlie such structural and kinetic studies, do not hold. Three new crystal structures of elastase complexed to chemically similar inhibitors with similar binding affinities reveal a diversity of binding modes as well as two new subsites on elastase. The existence of multiple binding sites and different binding modes for such similar inhibitors indicates that researchers must proceed with caution when using kinetics to map out protein subsites.
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*[[Elastase 3D structures|Elastase 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1ELB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELB OCA].
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__TOC__
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</StructureSection>
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==Reference==
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[[Category: Large Structures]]
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Analogous inhibitors of elastase do not always bind analogously., Mattos C, Rasmussen B, Ding X, Petsko GA, Ringe D, Nat Struct Biol. 1994 Jan;1(1):55-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7656008 7656008]
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[[Category: Pancreatic elastase]]
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[[Category: Single protein]]
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Ding, X.]]
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[[Category: Ding X]]
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[[Category: Mattos, C.]]
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[[Category: Mattos C]]
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[[Category: Petsko, G A.]]
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[[Category: Petsko GA]]
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[[Category: Rasmussen, B.]]
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[[Category: Rasmussen B]]
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[[Category: Ringe, D.]]
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[[Category: Ringe D]]
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[[Category: CA]]
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[[Category: SO4]]
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[[Category: hydrolase(serine proteinase)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:57:10 2008''
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Current revision

Analogous inhibitors of elastase do not always bind analogously

PDB ID 1elb

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