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1elw

From Proteopedia

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[[Image:1elw.gif|left|200px]]
 
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{{Structure
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==Crystal structure of the TPR1 domain of HOP in complex with a HSC70 peptide==
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|PDB= 1elw |SIZE=350|CAPTION= <scene name='initialview01'>1elw</scene>, resolution 1.60&Aring;
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<StructureSection load='1elw' size='340' side='right'caption='[[1elw]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene> and <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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<table><tr><td colspan='2'>[[1elw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ELW FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1elw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1elw OCA], [https://pdbe.org/1elw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1elw RCSB], [https://www.ebi.ac.uk/pdbsum/1elw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1elw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/STIP1_HUMAN STIP1_HUMAN] Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/el/1elw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1elw ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of the TPR1 domain of HOP in complex with a HSC70 peptide'''
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==See Also==
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*[[HOP protein|HOP protein]]
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__TOC__
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==Overview==
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</StructureSection>
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The adaptor protein Hop mediates the association of the molecular chaperones Hsp70 and Hsp90. The TPR1 domain of Hop specifically recognizes the C-terminal heptapeptide of Hsp70 while the TPR2A domain binds the C-terminal pentapeptide of Hsp90. Both sequences end with the motif EEVD. The crystal structures of the TPR-peptide complexes show the peptides in an extended conformation, spanning a groove in the TPR domains. Peptide binding is mediated by electrostatic interactions with the EEVD motif, with the C-terminal aspartate acting as a two-carboxylate anchor, and by hydrophobic interactions with residues upstream of EEVD. The hydrophobic contacts with the peptide are critical for specificity. These results explain how TPR domains participate in the ordered assembly of Hsp70-Hsp90 multichaperone complexes.
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==About this Structure==
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1ELW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELW OCA].
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==Reference==
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Structure of TPR domain-peptide complexes: critical elements in the assembly of the Hsp70-Hsp90 multichaperone machine., Scheufler C, Brinker A, Bourenkov G, Pegoraro S, Moroder L, Bartunik H, Hartl FU, Moarefi I, Cell. 2000 Apr 14;101(2):199-210. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10786835 10786835]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Brinker, A.]]
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[[Category: Brinker A]]
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[[Category: Hartl, F U.]]
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[[Category: Hartl FU]]
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[[Category: Moarefi, I.]]
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[[Category: Moarefi I]]
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[[Category: Scheufler, C.]]
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[[Category: Scheufler C]]
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[[Category: NI]]
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[[Category: TRS]]
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[[Category: helical repeat]]
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[[Category: hop]]
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[[Category: hsc70]]
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[[Category: hsp70]]
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[[Category: peptide-complex]]
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[[Category: protein binding]]
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[[Category: tpr-domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:57:22 2008''
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Current revision

Crystal structure of the TPR1 domain of HOP in complex with a HSC70 peptide

PDB ID 1elw

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