1eoo

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[[Image:1eoo.gif|left|200px]]
 
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{{Structure
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==ECORV BOUND TO COGNATE DNA==
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|PDB= 1eoo |SIZE=350|CAPTION= <scene name='initialview01'>1eoo</scene>, resolution 2.16&Aring;
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<StructureSection load='1eoo' size='340' side='right'caption='[[1eoo]], [[Resolution|resolution]] 2.16&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1eoo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EOO FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.16&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eoo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eoo OCA], [https://pdbe.org/1eoo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eoo RCSB], [https://www.ebi.ac.uk/pdbsum/1eoo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eoo ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/T2E5_ECOLX T2E5_ECOLX] Recognizes the double-stranded sequence GATATC and cleaves after T-3.
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'''ECORV BOUND TO COGNATE DNA'''
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==See Also==
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Two new high-resolution cocrystal structures of EcoRV endonuclease bound to DNA show that a large variation in DNA-bending angles is sampled in the ground state binary complex. Together with previous structures, these data reveal a contiguous series of protein conformational states delineating a specific trajectory for the induced-fit pathway. Rotation of the DNA-binding domains, together with movements of two symmetry-related helices binding in the minor groove, causes base unstacking at a key base-pair step and propagates structural changes that assemble the active sites. These structures suggest a complex mechanism for DNA bending that depends on forces generated by interacting protein segments, and on selective neutralization of phosphate charges along the inner face of the bent double helix.
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==About this Structure==
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1EOO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOO OCA].
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==Reference==
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Crystallographic snapshots along a protein-induced DNA-bending pathway., Horton NC, Perona JJ, Proc Natl Acad Sci U S A. 2000 May 23;97(11):5729-34. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10801972 10801972]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Type II site-specific deoxyribonuclease]]
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[[Category: Horton NC]]
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[[Category: Horton, N C.]]
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[[Category: Perona JJ]]
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[[Category: Perona, J J.]]
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[[Category: endonuclease]]
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[[Category: induced fit]]
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[[Category: protein-dna recognition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:58:32 2008''
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Current revision

ECORV BOUND TO COGNATE DNA

PDB ID 1eoo

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