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1eqp

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[[Image:1eqp.gif|left|200px]]
 
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{{Structure
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==EXO-B-(1,3)-GLUCANASE FROM CANDIDA ALBICANS==
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|PDB= 1eqp |SIZE=350|CAPTION= <scene name='initialview01'>1eqp</scene>, resolution 1.9&Aring;
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<StructureSection load='1eqp' size='340' side='right'caption='[[1eqp]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1eqp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EQP FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Glucan_1,3-beta-glucosidase Glucan 1,3-beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.58 3.2.1.58]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eqp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eqp OCA], [https://pdbe.org/1eqp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eqp RCSB], [https://www.ebi.ac.uk/pdbsum/1eqp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eqp ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EXG1_CANAL EXG1_CANAL] Major glucan 1,3-beta-glucosidase required for cell wall integrity. Beta-glucanases participate in the metabolism of beta-glucan, the main structural component of the cell wall. Can also function biosynthetically as a transglycosylase. Functions to deliver glucan from the cell to the extracellular matrix. Does not appear to impact cell wall glucan content of biofilm cells, nor is it necessary for filamentation or biofilm formation. Involved in cell-substrate and cell-cell adhesion. Adhesion to host-cell surfaces is the first critical step during mucosal infection. XOG1 is target of human antimicrobial peptide LL-37 for inhibition of cell adhesion.<ref>PMID:21713010</ref> <ref>PMID:22876186</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eq/1eqp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eqp ConSurf].
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<div style="clear:both"></div>
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'''EXO-B-(1,3)-GLUCANASE FROM CANDIDA ALBICANS'''
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==See Also==
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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== References ==
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==Overview==
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<references/>
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In some species of Candida the CUG codon is encoded as serine and not leucine. In the case of the exo-beta-1,3-glucanase from the pathogenic fungus C. albicans there are two such translational events, one in the prepro-leader sequence and the other at residue 64. Overexpression of active mature enzyme in a yeast host indicated that these two positions are tolerant to substitution. By comparing the crystal structure of the recombinant protein with that of the native (presented here), it is seen how either serine or leucine can be accommodated at position 64. Examination of the relatively few solved protein structures from C. albicans indicates that other CUG encoded serines are also found at non-essential surface sites. However such codon usage is rare in C. albicans, in contrast to C. rugosa, with direct implications for respective recombinant protein production.
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__TOC__
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</StructureSection>
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==About this Structure==
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1EQP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQP OCA].
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==Reference==
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Minor structural consequences of alternative CUG codon usage (Ser for Leu) in Candida albicans exoglucanase., Cutfield JF, Sullivan PA, Cutfield SM, Protein Eng. 2000 Oct;13(10):735-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11112513 11112513]
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[[Category: Candida albicans]]
[[Category: Candida albicans]]
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[[Category: Glucan 1,3-beta-glucosidase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Cutfield JF]]
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[[Category: Cutfield, J F.]]
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[[Category: Cutfield SM]]
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[[Category: Cutfield, S M.]]
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[[Category: Sullivan PA]]
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[[Category: Sullivan, P A.]]
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[[Category: alternative codon usage]]
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[[Category: candida albican]]
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[[Category: exoglucanase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:59:07 2008''
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Current revision

EXO-B-(1,3)-GLUCANASE FROM CANDIDA ALBICANS

PDB ID 1eqp

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