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1ewr

From Proteopedia

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[[Image:1ewr.gif|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF TAQ MUTS==
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|PDB= 1ewr |SIZE=350|CAPTION= <scene name='initialview01'>1ewr</scene>, resolution 3.19&Aring;
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<StructureSection load='1ewr' size='340' side='right'caption='[[1ewr]], [[Resolution|resolution]] 3.19&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1ewr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EWR FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.19&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ewr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ewr OCA], [https://pdbe.org/1ewr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ewr RCSB], [https://www.ebi.ac.uk/pdbsum/1ewr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ewr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MUTS_THEAQ MUTS_THEAQ] This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/1ewr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ewr ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF TAQ MUTS'''
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==See Also==
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*[[DNA mismatch repair protein 3D structures|DNA mismatch repair protein 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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DNA mismatch repair is critical for increasing replication fidelity in organisms ranging from bacteria to humans. MutS protein, a member of the ABC ATPase superfamily, recognizes mispaired and unpaired bases in duplex DNA and initiates mismatch repair. Mutations in human MutS genes cause a predisposition to hereditary nonpolyposis colorectal cancer as well as sporadic tumours. Here we report the crystal structures of a MutS protein and a complex of MutS with a heteroduplex DNA containing an unpaired base. The structures reveal the general architecture of members of the MutS family, an induced-fit mechanism of recognition between four domains of a MutS dimer and a heteroduplex kinked at the mismatch, a composite ATPase active site composed of residues from both MutS subunits, and a transmitter region connecting the mismatch-binding and ATPase domains. The crystal structures also provide a molecular framework for understanding hereditary nonpolyposis colorectal cancer mutations and for postulating testable roles of MutS.
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[[Category: Large Structures]]
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==About this Structure==
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1EWR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWR OCA].
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==Reference==
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Crystal structures of mismatch repair protein MutS and its complex with a substrate DNA., Obmolova G, Ban C, Hsieh P, Yang W, Nature. 2000 Oct 12;407(6805):703-10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11048710 11048710]
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[[Category: Single protein]]
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[[Category: Thermus aquaticus]]
[[Category: Thermus aquaticus]]
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[[Category: Ban, C.]]
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[[Category: Ban C]]
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[[Category: Hsieh, P.]]
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[[Category: Hsieh P]]
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[[Category: Obmolova, G.]]
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[[Category: Obmolova G]]
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[[Category: Yang, W.]]
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[[Category: Yang W]]
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[[Category: atp-binding]]
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[[Category: dna repair]]
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[[Category: dna-binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:01:18 2008''
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Current revision

CRYSTAL STRUCTURE OF TAQ MUTS

PDB ID 1ewr

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