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1eyg

From Proteopedia

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[[Image:1eyg.gif|left|200px]]
 
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{{Structure
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==Crystal structure of chymotryptic fragment of E. coli ssb bound to two 35-mer single strand DNAS==
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|PDB= 1eyg |SIZE=350|CAPTION= <scene name='initialview01'>1eyg</scene>, resolution 2.80&Aring;
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<StructureSection load='1eyg' size='340' side='right'caption='[[1eyg]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1eyg]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EYG FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eyg OCA], [https://pdbe.org/1eyg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eyg RCSB], [https://www.ebi.ac.uk/pdbsum/1eyg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eyg ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SSB_ECOLI SSB_ECOLI] This protein is essential for replication of the chromosomes and its single-stranded DNA phages. It is also involved in DNA recombination and repair.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ey/1eyg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eyg ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of chymotryptic fragment of E. coli ssb bound to two 35-mer single strand DNAS'''
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==See Also==
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*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The structure of the homotetrameric DNA binding domain of the single stranded DNA binding protein from Escherichia coli (Eco SSB) bound to two 35-mer single stranded DNAs was determined to a resolution of 2.8 A. This structure describes the vast network of interactions that results in the extensive wrapping of single stranded DNA around the SSB tetramer and suggests a structural basis for its various binding modes.
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==About this Structure==
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1EYG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYG OCA].
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==Reference==
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Structure of the DNA binding domain of E. coli SSB bound to ssDNA., Raghunathan S, Kozlov AG, Lohman TM, Waksman G, Nat Struct Biol. 2000 Aug;7(8):648-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10932248 10932248]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Raghunathan, S.]]
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[[Category: Raghunathan S]]
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[[Category: Waksman, G.]]
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[[Category: Waksman G]]
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[[Category: protein-dna complex; ob fold; se-met; mad phasing; ssb; binding mode]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:01:55 2008''
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Current revision

Crystal structure of chymotryptic fragment of E. coli ssb bound to two 35-mer single strand DNAS

PDB ID 1eyg

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