1f75

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[[Image:1f75.gif|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF UNDECAPRENYL DIPHOSPHATE SYNTHASE FROM MICROCOCCUS LUTEUS B-P 26==
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|PDB= 1f75 |SIZE=350|CAPTION= <scene name='initialview01'>1f75</scene>, resolution 2.2&Aring;
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<StructureSection load='1f75' size='340' side='right'caption='[[1f75]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1f75]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Micrococcus_luteus Micrococcus luteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F75 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F75 FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Di-trans,poly-cis-decaprenylcistransferase Di-trans,poly-cis-decaprenylcistransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.31 2.5.1.31]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f75 OCA], [https://pdbe.org/1f75 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f75 RCSB], [https://www.ebi.ac.uk/pdbsum/1f75 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f75 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UPPS_MICLU UPPS_MICLU] Generates ditrans,octacis-undecaprenyl pyrophosphate (UPP) from isopentenyl pyrophosphate (IPP) and farnesyl diphosphate. UPP is the precursor of glycosyl carrier lipid in the biosynthesis of bacterial cell wall polysaccharide components such as peptidoglycan and lipopolysaccharide.<ref>PMID:9677368</ref> <ref>PMID:3182755</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f7/1f75_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f75 ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF UNDECAPRENYL DIPHOSPHATE SYNTHASE FROM MICROCOCCUS LUTEUS B-P 26'''
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==See Also==
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*[[Undecaprenyl pyrophosphate synthase|Undecaprenyl pyrophosphate synthase]]
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== References ==
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==Overview==
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<references/>
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Undecaprenyl diphosphate synthase (UPS) catalyzes the cis-prenyl chain elongation onto trans, trans-farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of bacterial cell walls. We report here the crystal structure of UPS as the only three-dimensional structure among cis-prenyl chain elongating enzymes. The structure is classified into a protein fold family and is completely different from the so-called "isoprenoid synthase fold" that is believed to be a common structure for the enzymes relating to isoprenoid biosynthesis. Conserved amino acid residues among cis-prenyl chain elongating enzymes are located around a large hydrophobic cleft in the UPS structure. A structural P-loop motif, which frequently appears in the various kinds of phosphate binding site, is found at the entrance of this cleft. The catalytic site is determined on the basis of these structural features, from which a possible reaction mechanism is proposed.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1F75 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Micrococcus_luteus Micrococcus luteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F75 OCA].
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==Reference==
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Crystal structure of cis-prenyl chain elongating enzyme, undecaprenyl diphosphate synthase., Fujihashi M, Zhang YW, Higuchi Y, Li XY, Koyama T, Miki K, Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4337-42. Epub 2001 Apr 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11287651 11287651]
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[[Category: Di-trans,poly-cis-decaprenylcistransferase]]
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[[Category: Micrococcus luteus]]
[[Category: Micrococcus luteus]]
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[[Category: Single protein]]
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[[Category: Fujihashi M]]
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[[Category: Fujihashi, M.]]
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[[Category: Higuchi Y]]
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[[Category: Higuchi, Y.]]
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[[Category: Koyama T]]
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[[Category: Koyama, T.]]
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[[Category: Li X-Y]]
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[[Category: Li, X Y.]]
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[[Category: Miki K]]
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[[Category: Miki, K.]]
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[[Category: Zhang Y-W]]
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[[Category: Zhang, Y W.]]
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[[Category: SO4]]
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[[Category: new fold for isoprenoid synthase]]
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[[Category: parallel beta sheet]]
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[[Category: peptidoglycan synthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:05:22 2008''
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Current revision

CRYSTAL STRUCTURE OF UNDECAPRENYL DIPHOSPHATE SYNTHASE FROM MICROCOCCUS LUTEUS B-P 26

PDB ID 1f75

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