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1faz

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[[Image:1faz.gif|left|200px]]
 
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{{Structure
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==THE CRYSTAL STRUCTURE OF PROKARYOTIC PHOSPHOLIPASE A2==
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|PDB= 1faz |SIZE=350|CAPTION= <scene name='initialview01'>1faz</scene>, resolution 1.40&Aring;
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<StructureSection load='1faz' size='340' side='right'caption='[[1faz]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1faz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_violaceoruber Streptomyces violaceoruber]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FAZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FAZ FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1faz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1faz OCA], [https://pdbe.org/1faz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1faz RCSB], [https://www.ebi.ac.uk/pdbsum/1faz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1faz ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6UV28_STRVN Q6UV28_STRVN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fa/1faz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1faz ConSurf].
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<div style="clear:both"></div>
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'''THE CRYSTAL STRUCTURE OF PROKARYOTIC PHOSPHOLIPASE A2'''
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==See Also==
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*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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In this study, the x-ray crystal structures of the calcium-free and calcium-bound forms of phospholipase A(2) (PLA(2)), produced extracellularly by Streptomyces violaceoruber, were determined by using the multiple isomorphous replacement and molecular replacement methods, respectively. The former and latter structures were refined to an R-factor of 18.8% at a 1.4-A resolution and an R-factor of 15.0% at a 1.6-A resolution, respectively. The overall structure of the prokaryotic PLA(2) exhibits a novel folding topology that demonstrates that it is completely distinct from those of eukaryotic PLA(2)s, which have been already determined by x-ray and NMR analyses. Furthermore, the coordination geometry of the calcium(II) ion apparently deviated from that of eukaryotic PLA(2)s. Regardless of the evolutionary divergence, the catalytic mechanism including the calcium(II) ion on secreted PLA(2) seems to be conserved between prokaryotic and eukaryotic cells. Demonstrating that the overall structure determined by x-ray analysis is almost the same as that determined by NMR analysis is useful to discuss the catalytic mechanism at the molecular level of the bacterial PLA(2).
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[[Category: Large Structures]]
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==About this Structure==
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1FAZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_violaceoruber Streptomyces violaceoruber]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FAZ OCA].
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==Reference==
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The crystal structure of prokaryotic phospholipase A2., Matoba Y, Katsube Y, Sugiyama M, J Biol Chem. 2002 May 31;277(22):20059-69. Epub 2002 Mar 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11897785 11897785]
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[[Category: Phospholipase A(2)]]
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[[Category: Single protein]]
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[[Category: Streptomyces violaceoruber]]
[[Category: Streptomyces violaceoruber]]
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[[Category: Matoba, Y.]]
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[[Category: Matoba Y]]
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[[Category: Sugiyama, M.]]
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[[Category: Sugiyama M]]
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[[Category: katsube, Y.]]
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[[Category: Katsube Y]]
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[[Category: phospholipase a2]]
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[[Category: prokaryote]]
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[[Category: streptomyces violaceoruber]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:06:44 2008''
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Current revision

THE CRYSTAL STRUCTURE OF PROKARYOTIC PHOSPHOLIPASE A2

PDB ID 1faz

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