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1ffn

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[[Image:1ffn.gif|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF MURINE CLASS I H-2DB COMPLEXED WITH PEPTIDE GP33(C9M)==
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|PDB= 1ffn |SIZE=350|CAPTION= <scene name='initialview01'>1ffn</scene>, resolution 2.70&Aring;
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<StructureSection load='1ffn' size='340' side='right'caption='[[1ffn]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1ffn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FFN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FFN FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ffn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ffn OCA], [https://pdbe.org/1ffn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ffn RCSB], [https://www.ebi.ac.uk/pdbsum/1ffn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ffn ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HA11_MOUSE HA11_MOUSE] Involved in the presentation of foreign antigens to the immune system.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ff/1ffn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ffn ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF MURINE CLASS I H-2DB COMPLEXED WITH PEPTIDE GP33(C9M)'''
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==See Also==
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*[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
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*[[MHC 3D structures|MHC 3D structures]]
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==Overview==
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*[[MHC I 3D structures|MHC I 3D structures]]
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TCR recognition of class I MHC is dependent on the composition of the antigenic peptide and the MHC. Single amino acid substitutions in either the MHC or the peptide may dramatically alter recognition. While the major interactions between TCR and the peptide/MHC complex appear to be focused on the complementarity-determining region (CDR)3, it is also clear from the cocrystal structure of class I MHC and TCR that the amino and carboxyl ends of the peptide may play a role through interactions with the CDR1. In this work we show that gp33 variants substituted at the peptidic termini at the putative CDR1 contact regions show improved recognition in B6 mice. The rank order of recognition is different using the P14 transgenic T cells, suggesting that one reason for improved recognition is a change in the TCR repertoire that recognizes the peptide. However, the affinity of the TCR by some of the peptide/MHC complex with increased recognition is improved, as shown by increased tetramer binding to P14 T cells. These substitutions at the termini of the peptide-binding cleft cause localized conformational changes as seen by changes in mAb binding and crystallographic structures. The different peptide structures also show different conformations in the center of the peptide, but these are shown to be energetically similar and thus most likely have no significance with respect to TCR recognition. Therefore, small conformational changes, localized to the CDR1 contact regions, may play a significant role in TCR recognition.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1FFN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FFN OCA].
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==Reference==
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Peptidic termini play a significant role in TCR recognition., Wang B, Sharma A, Maile R, Saad M, Collins EJ, Frelinger JA, J Immunol. 2002 Sep 15;169(6):3137-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12218131 12218131]
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Collins EJ]]
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[[Category: Collins, E J.]]
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[[Category: Frelinger JA]]
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[[Category: Frelinger, J A.]]
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[[Category: Maile R]]
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[[Category: Maile, R.]]
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[[Category: Saad M]]
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[[Category: Saad, M.]]
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[[Category: Sharma A]]
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[[Category: Sharma, A.]]
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[[Category: Wang B]]
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[[Category: Wang, B.]]
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[[Category: crystallography]]
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[[Category: major histocompatibility complex]]
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[[Category: peptide binding]]
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[[Category: t cell receptor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:08:25 2008''
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Current revision

CRYSTAL STRUCTURE OF MURINE CLASS I H-2DB COMPLEXED WITH PEPTIDE GP33(C9M)

PDB ID 1ffn

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