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1fjg

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[[Image:1fjg.gif|left|200px]]
 
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{{Structure
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==STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTICS STREPTOMYCIN, SPECTINOMYCIN, AND PAROMOMYCIN==
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|PDB= 1fjg |SIZE=350|CAPTION= <scene name='initialview01'>1fjg</scene>, resolution 3.000&Aring;
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<StructureSection load='1fjg' size='340' side='right'caption='[[1fjg]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=SCM:SPECTINOMYCIN'>SCM</scene> and <scene name='pdbligand=SRY:STREPTOMYCIN'>SRY</scene>
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<table><tr><td colspan='2'>[[1fjg]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. The October 2000 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Ribosome'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2000_10 10.2210/rcsb_pdb/mom_2000_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FJG FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=SCM:SPECTINOMYCIN'>SCM</scene>, <scene name='pdbligand=SRY:STREPTOMYCIN'>SRY</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fjg OCA], [https://pdbe.org/1fjg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fjg RCSB], [https://www.ebi.ac.uk/pdbsum/1fjg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fjg ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS11_THET8 RS11_THET8] Located on the upper part of the platform of the 30S subunit, where it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome, where it interacts both with the Shine-Dalgarno helix and mRNA.[HAMAP-Rule:MF_01310]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fj/1fjg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fjg ConSurf].
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<div style="clear:both"></div>
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'''STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTICS STREPTOMYCIN, SPECTINOMYCIN, AND PAROMOMYCIN'''
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==See Also==
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*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
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*[[Ribosome|Ribosome]]
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==Overview==
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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The 30S ribosomal subunit has two primary functions in protein synthesis. It discriminates against aminoacyl transfer RNAs that do not match the codon of messenger RNA, thereby ensuring accuracy in translation of the genetic message in a process called decoding. Also, it works with the 50S subunit to move the tRNAs and associated mRNA by precisely one codon, in a process called translocation. Here we describe the functional implications of the high-resolution 30S crystal structure presented in the accompanying paper, and infer details of the interactions between the 30S subunit and its tRNA and mRNA ligands. We also describe the crystal structure of the 30S subunit complexed with the antibiotics paromomycin, streptomycin and spectinomycin, which interfere with decoding and translocation. This work reveals the structural basis for the action of these antibiotics, and leads to a model for the role of the universally conserved 16S RNA residues A1492 and A1493 in the decoding process.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1FJG is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. The following page contains interesting information on the relation of 1FJG with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb10_1.html Ribosome]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJG OCA].
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[[Category: RCSB PDB Molecule of the Month]]
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==Reference==
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Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics., Carter AP, Clemons WM, Brodersen DE, Morgan-Warren RJ, Wimberly BT, Ramakrishnan V, Nature. 2000 Sep 21;407(6802):340-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11014183 11014183]
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[[Category: Protein complex]]
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[[Category: Ribosome]]
[[Category: Ribosome]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Brodersen, D E.]]
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[[Category: Brodersen DE]]
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[[Category: Carter, A P.]]
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[[Category: Carter AP]]
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[[Category: Jr., W M.Clemons.]]
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[[Category: Clemons Jr WM]]
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[[Category: Morgan-Warren, R J.]]
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[[Category: Morgan-Warren RJ]]
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[[Category: Ramakrishnan, V.]]
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[[Category: Ramakrishnan V]]
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[[Category: Wimberly, B T.]]
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[[Category: Wimberly BT]]
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[[Category: MG]]
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[[Category: PAR]]
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[[Category: SCM]]
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[[Category: SRY]]
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[[Category: ZN]]
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[[Category: 30s ribosomal subunit]]
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[[Category: antibiotic]]
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[[Category: paromomycin]]
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[[Category: ribosome]]
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[[Category: spectinomycin]]
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[[Category: streptomycin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:09:52 2008''
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Current revision

STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTICS STREPTOMYCIN, SPECTINOMYCIN, AND PAROMOMYCIN

PDB ID 1fjg

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