1rmn

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==A THREE-DIMENSIONAL MODEL FOR THE HAMMERHEAD RIBOZYME BASED ON FLUORESCENCE MEASUREMENTS==
==A THREE-DIMENSIONAL MODEL FOR THE HAMMERHEAD RIBOZYME BASED ON FLUORESCENCE MEASUREMENTS==
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<StructureSection load='1rmn' size='340' side='right' caption='[[1rmn]]' scene=''>
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<StructureSection load='1rmn' size='340' side='right'caption='[[1rmn]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[1rmn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RMN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RMN FirstGlance]. <br>
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<table><tr><td colspan='2'>[[1rmn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RMN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RMN FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rmn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rmn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rmn RCSB], [http://www.ebi.ac.uk/pdbsum/1rmn PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Fluorescence transfer</td></tr>
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<table>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rmn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rmn OCA], [https://pdbe.org/1rmn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rmn RCSB], [https://www.ebi.ac.uk/pdbsum/1rmn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rmn ProSAT]</span></td></tr>
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<div style="background-color:#fffaf0;">
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</table>
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== Publication Abstract from PubMed ==
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For the understanding of the catalytic function of the RNA hammerhead ribozyme, a three-dimensional model is essential but neither a crystal nor a solution structure has been available. Fluorescence resonance energy transfer (FRET) was used to study the structure of the ribozyme in solution in order to establish the relative spatial orientation of the three constituent Watson-Crick base-paired helical segments. Synthetic constructs were labeled with the fluorescence donor (5-carboxyfluorescein) and acceptor (5-carboxytetramethylrhodamine) located at the ends of the strands constituting the ribozyme molecule. The acceptor helix in helix pairs I and III and in II and III was varied in length from 5 to 11 and 5 to 9 base pairs, respectively, and the FRET efficiencies were determined and correlated with a reference set of labeled RNA duplexes. The FRET efficiencies were predicted on the basis of vector algebra analysis, as a function of the relative helical orientations in the ribozyme constructs, and compared with experimental values. The data were consistent with a Y-shaped arrangement of the ribozyme with helices I and II in close proximity and helix III pointing away. These orientational constraints were used for molecular modeling of a three-dimensional structure of the complete ribozyme.
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A three-dimensional model for the hammerhead ribozyme based on fluorescence measurements.,Tuschl T, Gohlke C, Jovin TM, Westhof E, Eckstein F Science. 1994 Nov 4;266(5186):785-9. PMID:7973630<ref>PMID:7973630</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[Ribozyme|Ribozyme]]
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*[[Ribozyme 3D structures|Ribozyme 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Eckstein, F.]]
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[[Category: Large Structures]]
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[[Category: Gohlke, C.]]
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[[Category: Eckstein F]]
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[[Category: Jovin, T M.]]
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[[Category: Gohlke C]]
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[[Category: Tuschl, T.]]
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[[Category: Jovin TM]]
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[[Category: Westhof, E.]]
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[[Category: Tuschl T]]
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[[Category: Westhof E]]

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A THREE-DIMENSIONAL MODEL FOR THE HAMMERHEAD RIBOZYME BASED ON FLUORESCENCE MEASUREMENTS

PDB ID 1rmn

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