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1fvu

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[[Image:1fvu.gif|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF BOTROCETIN==
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|PDB= 1fvu |SIZE=350|CAPTION= <scene name='initialview01'>1fvu</scene>, resolution 1.80&Aring;
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<StructureSection load='1fvu' size='340' side='right'caption='[[1fvu]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
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<table><tr><td colspan='2'>[[1fvu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bothrops_jararaca Bothrops jararaca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FVU FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fvu OCA], [https://pdbe.org/1fvu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fvu RCSB], [https://www.ebi.ac.uk/pdbsum/1fvu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fvu ProSAT]</span></td></tr>
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</table>
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'''CRYSTAL STRUCTURE OF BOTROCETIN'''
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== Function ==
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[https://www.uniprot.org/uniprot/SLEA_BOTJA SLEA_BOTJA] Snaclec that binds to von Willebrand factor (VWF) and induces its interaction with GPIbalpha (GP1BA) (via the vWF A1 domain), resulting in platelet aggregation.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The binding of von Willebrand factor (vWF) to the platelet receptor, glycoprotein (GP) Ib-IX-V complex, has a key role in the initiation of thrombus formation and is regulated by interactions with extracellular matrix components under the influence of hemodynamic forces. To a certain extent, these effects can be mimicked in vitro by two nonphysiologic modulators, ristocetin and botrocetin. The latter, isolated from the venom of the snake Bothrops jararaca, is a 31-kDa heterodimeric protein that forms a soluble complex with vWF. As an initial step toward understanding the mechanisms that regulate vWF function, we have solved the crystal structure of botrocetin at 1.8 A resolution. Botrocetin exhibits homology with other snake proteins, but contains only one metal binding site as compared to two in Factor IX binding protein and Factor IX/X binding protein and none in flavocetin. A distinctive feature of botrocetin is the presence of a negatively charged surface that may play a role in the association with the vWF A1 domain.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fv/1fvu_consurf.spt"</scriptWhenChecked>
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1FVU is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bothrops_jararaca Bothrops jararaca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FVU OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of the von Willebrand factor modulator botrocetin., Sen U, Vasudevan S, Subbarao G, McClintock RA, Celikel R, Ruggeri ZM, Varughese KI, Biochemistry. 2001 Jan 16;40(2):345-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11148028 11148028]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fvu ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bothrops jararaca]]
[[Category: Bothrops jararaca]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Celikel, R.]]
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[[Category: Celikel R]]
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[[Category: McClintoc, R A.]]
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[[Category: McClintoc RA]]
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[[Category: Ruggeri, Z M.]]
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[[Category: Ruggeri ZM]]
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[[Category: Sen, U.]]
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[[Category: Sen U]]
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[[Category: Subbarao, G.]]
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[[Category: Subbarao G]]
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[[Category: Varughese, K I.]]
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[[Category: Varughese KI]]
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[[Category: Vasudevan, S.]]
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[[Category: Vasudevan S]]
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[[Category: MG]]
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[[Category: c-type lectin]]
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[[Category: loop exchanged dimer]]
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[[Category: metal-binding]]
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[[Category: von willbrand factor modulator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:14:41 2008''
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Current revision

CRYSTAL STRUCTURE OF BOTROCETIN

PDB ID 1fvu

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