1fw8

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[[Image:1fw8.gif|left|200px]]
 
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{{Structure
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==CIRCULARLY PERMUTED PHOSPHOGLYCERATE KINASE FROM YEAST: PGK P72==
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|PDB= 1fw8 |SIZE=350|CAPTION= <scene name='initialview01'>1fw8</scene>, resolution 2.3&Aring;
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<StructureSection load='1fw8' size='340' side='right'caption='[[1fw8]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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<table><tr><td colspan='2'>[[1fw8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FW8 FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Phosphoglycerate_kinase Phosphoglycerate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.3 2.7.2.3]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw8 OCA], [https://pdbe.org/1fw8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fw8 RCSB], [https://www.ebi.ac.uk/pdbsum/1fw8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fw8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PGK_YEAST PGK_YEAST]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fw/1fw8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fw8 ConSurf].
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<div style="clear:both"></div>
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'''CIRCULARLY PERMUTED PHOSPHOGLYCERATE KINASE FROM YEAST: PGK P72'''
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==See Also==
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*[[Phosphoglycerate kinase 3D structures|Phosphoglycerate kinase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The crystallographic structure of a circularly permuted form of yeast PGK, 72p yPGK, has been determined to a resolution of 2.3 A by molecular replacement. In this engineered protein, the C- and N-terminal residues of the wild-type protein are directly connected by a peptide bond and new N- and C-terminal residues are located within the N-terminal domain. The overall fold of the protein is very similar to that of the wild-type protein, directly demonstrating that the continuity of a folding unit is not relevant to the folding process of the whole protein. Only limited structural changes were observed: these were in the regions associated with the new connection, in a long flexible loop in the permuted domain and in the vicinity of Arg38, a functionally important residue. The relative positions of the two domains suggested that this permuted protein adopts one of the most open/twisted conformations seen amongst PGKs of known structure. The effect of the mutation on the functional properties is more easily accounted for by a restriction of hinge-bending motion than by structural changes in the protein.
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[[Category: Large Structures]]
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[[Category: Saccharomyces cerevisiae S288C]]
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==About this Structure==
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[[Category: Bizebard T]]
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1FW8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW8 OCA].
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[[Category: Desmadril M]]
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[[Category: Minard P]]
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==Reference==
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[[Category: Ritco-Vonsovici M]]
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Structure of a circularly permuted phosphoglycerate kinase., Tougard P, Bizebard T, Ritco-Vonsovici M, Minard P, Desmadril M, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2018-23. Epub 2002, Nov 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12454459 12454459]
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[[Category: Tougard P]]
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[[Category: Phosphoglycerate kinase]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Bizebard, T.]]
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[[Category: Desmadril, M.]]
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[[Category: Minard, P.]]
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[[Category: Ritco-Vonsovici, M.]]
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[[Category: Tougard, P.]]
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[[Category: ACE]]
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[[Category: GOL]]
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[[Category: glycolysis]]
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[[Category: kinase]]
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[[Category: mutant]]
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[[Category: permutation]]
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[[Category: permuted sequence]]
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[[Category: pgk]]
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[[Category: phosphoglycerate kinase]]
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[[Category: phosphotransferase]]
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[[Category: protein folding]]
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[[Category: two-domain protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:14:47 2008''
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Current revision

CIRCULARLY PERMUTED PHOSPHOGLYCERATE KINASE FROM YEAST: PGK P72

PDB ID 1fw8

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