This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1fxh

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:20, 7 February 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1fxh.jpg|left|200px]]
 
-
{{Structure
+
==MUTANT OF PENICILLIN ACYLASE IMPAIRED IN CATALYSIS WITH PHENYLACETIC ACID IN THE ACTIVE SITE==
-
|PDB= 1fxh |SIZE=350|CAPTION= <scene name='initialview01'>1fxh</scene>, resolution 1.97&Aring;
+
<StructureSection load='1fxh' size='340' side='right'caption='[[1fxh]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=PAC:2-PHENYLACETIC ACID'>PAC</scene>
+
<table><tr><td colspan='2'>[[1fxh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FXH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FXH FirstGlance]. <br>
-
|ACTIVITY= [http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11]
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.97&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PAC:2-PHENYLACETIC+ACID'>PAC</scene></td></tr>
-
}}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fxh OCA], [https://pdbe.org/1fxh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fxh RCSB], [https://www.ebi.ac.uk/pdbsum/1fxh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fxh ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PAC_ECOLX PAC_ECOLX]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fx/1fxh_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fxh ConSurf].
 +
<div style="clear:both"></div>
-
'''MUTANT OF PENICILLIN ACYLASE IMPAIRED IN CATALYSIS WITH PHENYLACETIC ACID IN THE ACTIVE SITE'''
+
==See Also==
-
 
+
*[[Penicillin acylase|Penicillin acylase]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
The binding of penicillin to penicillin acylase was studied by X-ray crystallography. The structure of the enzyme-substrate complex was determined after soaking crystals of an inactive betaN241A penicillin acylase mutant with penicillin G. Binding of the substrate induces a conformational change, in which the side chains of alphaF146 and alphaR145 move away from the active site, which allows the enzyme to accommodate penicillin G. In the resulting structure, the beta-lactam binding site is formed by the side chains of alphaF146 and betaF71, which have van der Waals interactions with the thiazolidine ring of penicillin G and the side chain of alphaR145 that is connected to the carboxylate group of the ligand by means of hydrogen bonding via two water molecules. The backbone oxygen of betaQ23 forms a hydrogen bond with the carbonyl oxygen of the phenylacetic acid moiety through a bridging water molecule. Kinetic studies revealed that the site-directed mutants alphaF146Y, alphaF146A and alphaF146L all show significant changes in their interaction with the beta-lactam substrates as compared with the wild type. The alphaF146Y mutant had the same affinity for 6-aminopenicillanic acid as the wild-type enzyme, but was not able to synthesize penicillin G from phenylacetamide and 6-aminopenicillanic acid. The alphaF146L and alphaF146A enzymes had a 3-5-fold decreased affinity for 6-aminopenicillanic acid, but synthesized penicillin G more efficiently than the wild type. The combined results of the structural and kinetic studies show the importance of alphaF146 in the beta-lactam binding site and provide leads for engineering mutants with improved synthetic properties.
+
-
 
+
-
==About this Structure==
+
-
1FXH is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FXH OCA].
+
-
 
+
-
==Reference==
+
-
Characterization of the beta-lactam binding site of penicillin acylase of Escherichia coli by structural and site-directed mutagenesis studies., Alkema WB, Hensgens CM, Kroezinga EH, de Vries E, Floris R, van der Laan JM, Dijkstra BW, Janssen DB, Protein Eng. 2000 Dec;13(12):857-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11239085 11239085]
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Penicillin amidase]]
+
[[Category: Large Structures]]
-
[[Category: Protein complex]]
+
[[Category: Alkema WB]]
-
[[Category: Alkema, W B.]]
+
[[Category: Dijkstra BW]]
-
[[Category: Dijkstra, B W.]]
+
[[Category: Floris R]]
-
[[Category: Floris, R.]]
+
[[Category: Hensgens CM]]
-
[[Category: Hensgens, C M.]]
+
[[Category: Janssen DB]]
-
[[Category: Janssen, D B.]]
+
[[Category: Kroezinga EH]]
-
[[Category: Kroezinga, E H.]]
+
[[Category: De Vries E]]
-
[[Category: Laan, J M.van der.]]
+
[[Category: Van der Laan JM]]
-
[[Category: Vries, E de.]]
+
-
[[Category: CA]]
+
-
[[Category: PAC]]
+
-
[[Category: ntn-hydrolase fold]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:15:18 2008''
+

Current revision

MUTANT OF PENICILLIN ACYLASE IMPAIRED IN CATALYSIS WITH PHENYLACETIC ACID IN THE ACTIVE SITE

PDB ID 1fxh

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1fxh"
Personal tools