2e5l

<StructureSection load='2e5l' size='340' side='right' caption='[[2e5l]], [[Resolution|resolution]] 3.30&Aring;' scene=''>

<table><tr><td colspan='2'>[[2e5l]] is a 23 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E5L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2E5L FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>

<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2e5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e5l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2e5l RCSB], [http://www.ebi.ac.uk/pdbsum/2e5l PDBsum], [http://www.topsan.org/Proteins/RSGI/2e5l TOPSAN]</span></td></tr>

<table>

<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e5/2e5l_consurf.spt"</scriptWhenChecked>

</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].

<div style="background-color:#fffaf0;">

== Publication Abstract from PubMed ==

In the initiation phase of bacterial translation, the 30S ribosomal subunit captures mRNA in preparation for binding with initiator tRNA. The purine-rich Shine-Dalgarno (SD) sequence, in the 5' untranslated region of the mRNA, anchors the 30S subunit near the start codon, via base pairing with an anti-SD (aSD) sequence at the 3' terminus of 16S rRNA. Here, we present the 3.3 A crystal structure of the Thermus thermophilus 30S subunit bound with an mRNA mimic. The duplex formed by the SD and aSD sequences is snugly docked in a "chamber" between the head and platform domains, demonstrating how the 30S subunit captures and stabilizes the otherwise labile SD helix. This location of the SD helix is suitable for the placement of the start codon AUG in the immediate vicinity of the mRNA channel, in agreement with reported crosslinks between the second position of the start codon and G1530 of 16S rRNA.

A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine-Dalgarno interaction.,Kaminishi T, Wilson DN, Takemoto C, Harms JM, Kawazoe M, Schluenzen F, Hanawa-Suetsugu K, Shirouzu M, Fucini P, Yokoyama S Structure. 2007 Mar;15(3):289-97. PMID:17355865<ref>PMID:17355865</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

</div>

*[[Ribosomal protein THX|Ribosomal protein THX]]

*[[Ribosome 3D structures|Ribosome 3D structures]]

[[Category: Fucini, P.]]

[[Category: Hanawa-Suetsugu, K.]]

[[Category: Harms, J M.]]

[[Category: Kaminishi, T.]]

[[Category: Kawazoe, M.]]

[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]

[[Category: Schluenzen, F.]]

[[Category: Shirouzu, M.]]

[[Category: Takemoto, C.]]

[[Category: Wilson, D N.]]

[[Category: Yokoyama, S.]]

[[Category: Translation initiation]]