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1g1i

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[[Image:1g1i.gif|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF THE OLIGOMERIZATION DOMAIN FROM ROTAVIRUS NSP4==
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|PDB= 1g1i |SIZE=350|CAPTION= <scene name='initialview01'>1g1i</scene>, resolution 2.00&Aring;
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<StructureSection load='1g1i' size='340' side='right'caption='[[1g1i]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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<table><tr><td colspan='2'>[[1g1i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Simian_rotavirus_A/SA11 Simian rotavirus A/SA11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G1I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G1I FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g1i OCA], [https://pdbe.org/1g1i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g1i RCSB], [https://www.ebi.ac.uk/pdbsum/1g1i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g1i ProSAT]</span></td></tr>
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</table>
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'''CRYSTAL STRUCTURE OF THE OLIGOMERIZATION DOMAIN FROM ROTAVIRUS NSP4'''
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== Function ==
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[https://www.uniprot.org/uniprot/NSP4_ROTS1 NSP4_ROTS1] Plays an essential role in the virus replication cycle by acting as a viroporin. Creates a pore in the host reticulum endoplasmic and as a consequence releases Ca(2+) in the cytoplasm of infected cell. In turn, high levels of cytoplasmic calcium trigger membrane trafficking and transport of viral ER-associated proteins to viroplasms, sites of viral genome replication and immature particle assembly.[HAMAP-Rule:MF_04091]<ref>PMID:21151776</ref> <ref>PMID:7637021</ref> <ref>PMID:9108087</ref> The secreted form acts as an enterotoxin that causes phospholipase C-dependent elevation of the intracellular calcium concentration in host intestinal mucosa cells. Increased concentration of intracellular calcium disrupts the cytoskeleton and the tight junctions, raising the paracellular permeability. Potentiates chloride ion secretion through a calcium ion-dependent signaling pathway, inducing age-dependent diarrhea. To perform this enterotoxigenic role in vivo, NSP4 is released from infected enterocytes in a soluble form capable of diffusing within the intestinal lumen and interacting with host plasma membrane receptors on neighboring epithelial cells such as integrins ITGA1/ITGB1 and ITGA2/ITGB1.[HAMAP-Rule:MF_04091]<ref>PMID:17035333</ref> <ref>PMID:18587047</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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During the maturation of rotaviral particles, non-structural protein 4 (NSP4) plays a critical role in the translocation of the immature capsid into the lumen of the endoplasmic reticulum. Full-length NSP4 and a 22 amino acid peptide (NSP4(114-135)) derived from this protein have been shown to induce diarrhea in young mice in an age-dependent manner, and may therefore be the agent responsible for rotavirally-induced symptoms. We have determined the crystal structure of the oligomerization domain of NSP4 which spans residues 95 to 137 (NSP4(95-137)). NSP4(95-137) self-associates into a parallel, tetrameric coiled-coil, with the hydrophobic core interrupted by three polar layers occupying a and d-heptad positions. Side-chains from two consecutive polar layers, consisting of four Gln123 and two of the four Glu120 residues, coordinate a divalent cation. Two independent structures built from MAD-phased data indicated the presence of a strontium and calcium ion bound at this site, respectively. This metal-binding site appears to play an important role in stabilizing the homo-tetramer, which has implications for the engagement of NSP4 as an enterotoxin.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g1/1g1i_consurf.spt"</scriptWhenChecked>
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1G1I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G1I OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of the oligomerization domain of NSP4 from rotavirus reveals a core metal-binding site., Bowman GD, Nodelman IM, Levy O, Lin SL, Tian P, Zamb TJ, Udem SA, Venkataraghavan B, Schutt CE, J Mol Biol. 2000 Dec 15;304(5):861-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11124032 11124032]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g1i ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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[[Category: Bowman, G D.]]
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== References ==
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[[Category: Nodelman, I M.]]
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<references/>
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[[Category: Schutt, C E.]]
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__TOC__
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[[Category: CA]]
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</StructureSection>
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[[Category: homo-tetramer]]
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[[Category: Large Structures]]
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[[Category: metal binding site]]
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[[Category: Simian rotavirus A/SA11]]
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[[Category: ns28]]
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[[Category: Bowman GD]]
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[[Category: parallel coiled-coil]]
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[[Category: Nodelman IM]]
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[[Category: Schutt CE]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:16:53 2008''
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Current revision

CRYSTAL STRUCTURE OF THE OLIGOMERIZATION DOMAIN FROM ROTAVIRUS NSP4

PDB ID 1g1i

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