1g8y

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[[Image:1g8y.gif|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF THE HEXAMERIC REPLICATIVE HELICASE REPA OF PLASMID RSF1010==
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|PDB= 1g8y |SIZE=350|CAPTION= <scene name='initialview01'>1g8y</scene>, resolution 2.40&Aring;
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<StructureSection load='1g8y' size='340' side='right'caption='[[1g8y]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1g8y]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G8Y FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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|GENE= REPA ON PLASMID INCQ RSF1010 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g8y OCA], [https://pdbe.org/1g8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g8y RCSB], [https://www.ebi.ac.uk/pdbsum/1g8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g8y ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/REPJ_ECOLX REPJ_ECOLX] This protein is involved in regulating the plasmid copy-number. Increasing the level of this protein results in a higher plasmid copy-number.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g8/1g8y_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g8y ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF THE HEXAMERIC REPLICATIVE HELICASE REPA OF PLASMID RSF1010'''
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==See Also==
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*[[Helicase 3D structures|Helicase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Unwinding of double-stranded DNA into single-stranded intermediates required for various fundamental life processes is catalyzed by helicases, a family of mono-, di- or hexameric motor proteins fueled by nucleoside triphosphate hydrolysis. The three-dimensional crystal structure of the hexameric helicase RepA encoded by plasmid RSF1010 has been determined by X-ray diffraction at 2.4 A resolution. The hexamer shows an annular structure with 6-fold rotational symmetry and a approximately 17 A wide central hole, suggesting that single-stranded DNA may be threaded during unwinding. Homologs of all five conserved sequence motifs of the DnaB-like helicase family are found in RepA, and the topography of the monomer resembles RecA and the helicase domain of the bacteriophage T7 gp4 protein. In a modeled complex, ATP molecules are located at the subunit interfaces and clearly define adenine-binding and ATPase catalytic sites formed by amino acid residues located on adjacent monomers; most remarkable is the "arginine finger" Arg207 contributing to the active site in the adjacent monomer. This arrangement of active-site residues suggests cooperativity between monomers in ATP hydrolysis and helicase activity of RepA. The mechanism of DNA unwinding remains elusive, as RepA is 6-fold symmetric, contrasting the recently published asymmetric structure of the bacteriophage T7 gp4 helicase domain.
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==About this Structure==
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1G8Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G8Y OCA].
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==Reference==
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Crystal structure of the hexameric replicative helicase RepA of plasmid RSF1010., Niedenzu T, Roleke D, Bains G, Scherzinger E, Saenger W, J Mol Biol. 2001 Feb 23;306(3):479-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11178907 11178907]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bains, G.]]
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[[Category: Bains G]]
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[[Category: Niedenzu, T.]]
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[[Category: Niedenzu T]]
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[[Category: Roeleke, D.]]
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[[Category: Roeleke D]]
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[[Category: Saenger, W.]]
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[[Category: Saenger W]]
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[[Category: Scherzinger, E.]]
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[[Category: Scherzinger E]]
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[[Category: p-loop]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:19:48 2008''
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Current revision

CRYSTAL STRUCTURE OF THE HEXAMERIC REPLICATIVE HELICASE REPA OF PLASMID RSF1010

PDB ID 1g8y

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