1ghs

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[[Image:1ghs.gif|left|200px]]
 
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{{Structure
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==THE THREE-DIMENSIONAL STRUCTURES OF TWO PLANT BETA-GLUCAN ENDOHYDROLASES WITH DISTINCT SUBSTRATE SPECIFICITIES==
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|PDB= 1ghs |SIZE=350|CAPTION= <scene name='initialview01'>1ghs</scene>, resolution 2.3&Aring;
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<StructureSection load='1ghs' size='340' side='right'caption='[[1ghs]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1ghs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GHS FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Glucan_endo-1,3-beta-D-glucosidase Glucan endo-1,3-beta-D-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.39 3.2.1.39]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ghs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ghs OCA], [https://pdbe.org/1ghs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ghs RCSB], [https://www.ebi.ac.uk/pdbsum/1ghs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ghs ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/E13B_HORVU E13B_HORVU] May provide a degree of protection against microbial invasion of germinated barley grain through its ability to degrade fungal cell wall polysaccharides.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gh/1ghs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ghs ConSurf].
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<div style="clear:both"></div>
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'''THE THREE-DIMENSIONAL STRUCTURES OF TWO PLANT BETA-GLUCAN ENDOHYDROLASES WITH DISTINCT SUBSTRATE SPECIFICITIES'''
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==See Also==
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The three-dimensional structures of (1--&gt;3)-beta-glucanase (EC 3.2.1.39) isoenzyme GII and (1--&gt;3,1--&gt;4)-beta-glucanase (EC 3.2.1.73) isoenzyme EII from barley have been determined by x-ray crystallography at 2.2- to 2.3-A resolution. The two classes of polysaccharide endohydrolase differ in their substrate specificity and function. Thus, the (1--&gt;3)-beta-glucanases, which are classified amongst the plant "pathogenesis-related proteins," can hydrolyze (1--&gt;3)- and (1--&gt;3,1--&gt;6)-beta-glucans of fungal cell walls and may therefore contribute to plant defense strategies, while the (1--&gt;3,1--&gt;4)-beta-glucanases function in plant cell wall hydrolysis during mobilization of the endosperm in germinating grain or during the growth of vegetative tissues. Both enzymes are alpha/beta-barrel structures. The catalytic amino acid residues are located within deep grooves which extend across the enzymes and which probably bind the substrates. Because the polypeptide backbones of the two enzymes are structurally very similar, the differences in their substrate specificities, and hence their widely divergent functions, have been acquired primarily by amino acid substitutions within the groove.
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==About this Structure==
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1GHS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GHS OCA].
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==Reference==
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Three-dimensional structures of two plant beta-glucan endohydrolases with distinct substrate specificities., Varghese JN, Garrett TP, Colman PM, Chen L, Hoj PB, Fincher GB, Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2785-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8146192 8146192]
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[[Category: Glucan endo-1,3-beta-D-glucosidase]]
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[[Category: Hordeum vulgare]]
[[Category: Hordeum vulgare]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Garrett, T P.J.]]
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[[Category: Garrett TPJ]]
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[[Category: Varghese, J N.]]
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[[Category: Varghese JN]]
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[[Category: hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:23:13 2008''
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Current revision

THE THREE-DIMENSIONAL STRUCTURES OF TWO PLANT BETA-GLUCAN ENDOHYDROLASES WITH DISTINCT SUBSTRATE SPECIFICITIES

PDB ID 1ghs

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