This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1h8g

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:29, 27 March 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1h8g.jpg|left|200px]]
 
-
{{Structure
+
==C-terminal domain of the major autolysin (C-LytA) from Streptococcus pneumoniae==
-
|PDB= 1h8g |SIZE=350|CAPTION= <scene name='initialview01'>1h8g</scene>, resolution 2.40&Aring;
+
<StructureSection load='1h8g' size='340' side='right'caption='[[1h8g]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=CHT:CHOLINE ION'>CHT</scene>
+
<table><tr><td colspan='2'>[[1h8g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H8G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H8G FirstGlance]. <br>
-
|ACTIVITY= [http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28]
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-
|GENE= LYTA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 Streptococcus pneumoniae])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CHT:CHOLINE+ION'>CHT</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-
}}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h8g OCA], [https://pdbe.org/1h8g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h8g RCSB], [https://www.ebi.ac.uk/pdbsum/1h8g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h8g ProSAT]</span></td></tr>
-
 
+
</table>
-
'''C-TERMINAL DOMAIN OF THE MAJOR AUTOLYSIN (C-LYTA) FROM STREPTOCOCCUS PNEUMONIAE'''
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/ALYS_STRPN ALYS_STRPN] Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Autolysin strictly depends on the presence of choline-containing cell walls for activity.
-
 
+
== Evolutionary Conservation ==
-
==Overview==
+
[[Image:Consurf_key_small.gif|200px|right]]
-
Choline binding proteins are virulence determinants present in several Gram-positive bacteria. Because anchorage of these proteins to the cell wall through their choline binding domain is essential for bacterial virulence, their release from the cell surface is considered a powerful target for a weapon against these pathogens. The first crystal structure of a choline binding domain, from the toxin-releasing enzyme pneumococcal major autolysin (LytA), reveals a novel solenoid fold consisting exclusively of beta-hairpins that stack to form a left-handed superhelix. This unique structure is maintained by choline molecules at the hydrophobic interface of consecutive hairpins and may be present in other choline binding proteins that share high homology to the repeated motif of the domain.
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==About this Structure==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h8/1h8g_consurf.spt"</scriptWhenChecked>
-
1H8G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H8G OCA].
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==Reference==
+
</jmolCheckbox>
-
A novel solenoid fold in the cell wall anchoring domain of the pneumococcal virulence factor LytA., Fernandez-Tornero C, Lopez R, Garcia E, Gimenez-Gallego G, Romero A, Nat Struct Biol. 2001 Dec;8(12):1020-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11694890 11694890]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h8g ConSurf].
-
[[Category: N-acetylmuramoyl-L-alanine amidase]]
+
<div style="clear:both"></div>
-
[[Category: Single protein]]
+
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Streptococcus pneumoniae]]
[[Category: Streptococcus pneumoniae]]
-
[[Category: Fernandez-Tornero, C.]]
+
[[Category: Fernandez-Tornero C]]
-
[[Category: Garcia, E.]]
+
[[Category: Garcia E]]
-
[[Category: Gimenez-Gallego, G.]]
+
[[Category: Gimenez-Gallego G]]
-
[[Category: Lopez, R.]]
+
[[Category: Lopez R]]
-
[[Category: Romero, A.]]
+
[[Category: Romero A]]
-
[[Category: CHT]]
+
-
[[Category: cell wall attachment]]
+
-
[[Category: choline-binding domain]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:33:43 2008''
+

Current revision

C-terminal domain of the major autolysin (C-LytA) from Streptococcus pneumoniae

PDB ID 1h8g

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1h8g"
Personal tools