This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1hcz

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:30, 27 March 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1hcz.gif|left|200px]]
 
-
{{Structure
+
==LUMEN-SIDE DOMAIN OF REDUCED CYTOCHROME F AT-35 DEGREES CELSIUS==
-
|PDB= 1hcz |SIZE=350|CAPTION= <scene name='initialview01'>1hcz</scene>, resolution 1.96&Aring;
+
<StructureSection load='1hcz' size='340' side='right'caption='[[1hcz]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene>
+
<table><tr><td colspan='2'>[[1hcz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brassica_rapa Brassica rapa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HCZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HCZ FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-
}}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hcz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hcz OCA], [https://pdbe.org/1hcz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hcz RCSB], [https://www.ebi.ac.uk/pdbsum/1hcz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hcz ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CYF_BRARR CYF_BRARR] Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hc/1hcz_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hcz ConSurf].
 +
<div style="clear:both"></div>
-
'''LUMEN-SIDE DOMAIN OF REDUCED CYTOCHROME F AT-35 DEGREES CELSIUS'''
+
==See Also==
-
 
+
*[[Cytochrome f 3D structures|Cytochrome f 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
The crystal structure of the 252-residue lumen-side domain of reduced cytochrome f, a subunit of the proton-pumping integral cytochrome b6f complex of oxygenic photosynthetic membranes, was determined to a resolution of 1.96 A from crystals cooled to -35 degrees. The model was refined to an R-factor of 15.8% with a 0.013-A RMS deviation of bond lengths from ideality. Compared to the structure of cytochrome f at 20 degrees, the structure at -35 degrees has a small change in relative orientation of the two folding domains and significantly lower isotropic temperature factors for protein atoms. The structure revealed an L-shaped array of five buried water molecules that extend in two directions from the N delta 1 of the heme ligand His 25. The longer branch extends 11 A within the large domain, toward Lys 66 in the prominent basic patch at the top of the large domain, which has been implicated in the interaction with the electron acceptor, plastocyanin. The water sites are highly occupied, and their temperature factors are comparable to those of protein atoms. Virtually all residues that form hydrogen bonds with the water chain are invariant among 13 known cytochrome f sequences. The water chain has many features that optimize it as a proton wire, including insulation from the protein medium. It is suggested that this chain may function as the lumen-side exit port for proton translocation by the cytochrome b6f complex.
+
-
 
+
-
==About this Structure==
+
-
1HCZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Brassica_rapa Brassica rapa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HCZ OCA].
+
-
 
+
-
==Reference==
+
-
The heme redox center of chloroplast cytochrome f is linked to a buried five-water chain., Martinez SE, Huang D, Ponomarev M, Cramer WA, Smith JL, Protein Sci. 1996 Jun;5(6):1081-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8762139 8762139]
+
[[Category: Brassica rapa]]
[[Category: Brassica rapa]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Cramer, W A.]]
+
[[Category: Cramer WA]]
-
[[Category: Huang, D.]]
+
[[Category: Huang D]]
-
[[Category: Martinez, S E.]]
+
[[Category: Martinez SE]]
-
[[Category: Smith, J L.]]
+
[[Category: Smith JL]]
-
[[Category: Szczepaniak, A.]]
+
[[Category: Szczepaniak A]]
-
[[Category: HEM]]
+
-
[[Category: chloroplast transmembrane]]
+
-
[[Category: cytochrome b6f complex]]
+
-
[[Category: electron transport]]
+
-
[[Category: photosynthesis]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:35:28 2008''
+

Current revision

LUMEN-SIDE DOMAIN OF REDUCED CYTOCHROME F AT-35 DEGREES CELSIUS

PDB ID 1hcz

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1hcz"
Personal tools