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2bk0

From Proteopedia

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Crystal structure of the major celery allergen Api G 1

Structural highlights

2bk0 is a 2 chain structure with sequence from Apium graveolens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.9Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ALL1_APIGR

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Many patients who have been sensitised to pollen, display allergic symptoms after ingestion of certain plant food such as fresh fruit, vegetables and nuts. The cause is the cross-reactivity between structurally very similar major plant allergens. In particular, allergy to celery is very frequently associated with birch and mugwort pollen sensitization, known as to the birch-mugwort-celery syndrome. The crystal structure of the major celery allergen Api g 1, a homologue of the major birch pollen allergen Bet v 1, has been determined to a resolution of 2.9 A. The structure of Api g 1 is very similar to that of Bet v 1 with major differences occurring in the segment comprised of residues 23-45, preceding the well conserved glycine-rich P-loop, as well as in loops beta3-beta4 and beta5-beta6. In particular, Api g 1 lacks E45, which has been shown to be a crucial residue for antibody recognition in the crystal complex of Bet v 1 with the Fab fragment of a murine monoclonal IgG (BV16) antibody. The absence of E45 and the structural differences in the preceding segment suggest that this region of the Api g 1 surface is probably not responsible for the observed cross-reactivity with Bet v 1. A detailed analysis of the molecular surface in combination with sequence alignment revealed three conserved surface patches which may account for cross-reactivity with Bet v 1. Several residues of Bet v 1 which have been shown by mutagenesis studies to be involved in IgE recognition belong to these conserved surface regions. The structure of Api g 1 and the related epitope analysis provides a molecular basis for a better understanding of allergen cross-reactivity and may lead to the development of hypoallergens which would allow a safer immunotherapy.

Crystal structure of the major celery allergen Api g 1: molecular analysis of cross-reactivity.,Schirmer T, Hoffimann-Sommergrube K, Susani M, Breiteneder H, Markovic-Housley Z J Mol Biol. 2005 Sep 2;351(5):1101-9. PMID:16051263^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schirmer T, Hoffimann-Sommergrube K, Susani M, Breiteneder H, Markovic-Housley Z. Crystal structure of the major celery allergen Api g 1: molecular analysis of cross-reactivity. J Mol Biol. 2005 Sep 2;351(5):1101-9. PMID:16051263 doi:10.1016/j.jmb.2005.06.054

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bk0"

@@ Line 1: / Line 1: @@
-==CRYSTAL STRUCTURE OF THE MAJOR CELERY ALLERGEN API G 1==
-<StructureSection load='2bk0' size='340' side='right' caption='[[2bk0]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
+==Crystal structure of the major celery allergen Api G 1==
+<StructureSection load='2bk0' size='340' side='right'caption='[[2bk0]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2bk0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Apium_graveolens Apium graveolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BK0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BK0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2bk0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Apium_graveolens Apium graveolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BK0 FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bk0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bk0 RCSB], [http://www.ebi.ac.uk/pdbsum/2bk0 PDBsum]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
-<table>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bk0 OCA], [https://pdbe.org/2bk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bk0 RCSB], [https://www.ebi.ac.uk/pdbsum/2bk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bk0 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ALL1_APIGR ALL1_APIGR]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/2bk0_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/2bk0_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bk0 ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 23: / Line 27: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2bk0" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
@@ Line 28: / Line 33: @@
 </StructureSection>
 [[Category: Apium graveolens]]
-[[Category: Breiteneder, H.]]
+[[Category: Large Structures]]
-[[Category: Hoffmann-Sommergruber, K.]]
+[[Category: Breiteneder H]]
-[[Category: Markovic-Housley, Z.]]
+[[Category: Hoffmann-Sommergruber K]]
-[[Category: Schirmer, T.]]
+[[Category: Markovic-Housley Z]]
-[[Category: Allergen]]
+[[Category: Schirmer T]]
-[[Category: Bet v 1-related protein]]
-[[Category: Cross reactive epitope]]
-[[Category: Major celery allergen api g 1]]
-[[Category: Pathogenesis-related protein]]
-[[Category: Plant defense]]

2bk0

From Proteopedia

Current revision

Crystal structure of the major celery allergen Api G 1

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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