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1hrj

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[[Image:1hrj.jpg|left|200px]]
 
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{{Structure
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==HUMAN RANTES, NMR, 13 STRUCTURES==
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|PDB= 1hrj |SIZE=350|CAPTION= <scene name='initialview01'>1hrj</scene>
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<StructureSection load='1hrj' size='340' side='right'caption='[[1hrj]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1hrj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HRJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HRJ FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hrj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hrj OCA], [https://pdbe.org/1hrj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hrj RCSB], [https://www.ebi.ac.uk/pdbsum/1hrj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hrj ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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'''HUMAN RANTES, NMR, 13 STRUCTURES'''
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[https://www.uniprot.org/uniprot/CCL5_HUMAN CCL5_HUMAN] Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.<ref>PMID:16791620</ref> <ref>PMID:1380064</ref> <ref>PMID:8525373</ref> <ref>PMID:9516414</ref> <ref>PMID:15923218</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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The solution structure of the chemokine RANTES (regulated on activation, normal T-cell expressed and secreted) has been determined using NMR spectroscopy. Backbone and side-chain 1H and 15N assignments have been obtained using a combination of two-dimensional homonuclear and three-dimensional heteronuclear spectra. Regular elements of secondary structure have been identified on the basis of a qualitative interpretation of NOE data, J(NH-H alpha) coupling constants, and amide exchange rates. Three-dimensional structures were calculated from a total of 2146 experimental restraints using a combination of distance geometry and simulated annealing protocols. For the 13 best structures the average backbone (N, C alpha, C) atomic rmsd from the mean coordinates for residues 5-65 is 0.64 A (+/- 0.14 A) for the dimer and 0.50 A (+/- 0.08 A) for the individual monomers. Each monomer consists of a three-stranded antiparallel beta-sheet (residues 26-30, 38-43, 48-51) in a Greek key motif with a C-terminal helix (56-65) packed across the sheet, an arrangement similar to the monomeric structure of other members of this chemokine family (IL-8, PF4, MGSA/Gro alpha, and MIP-1 beta). Overall, the RANTES dimer resembles that previously reported for MIP-1 beta.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hr/1hrj_consurf.spt"</scriptWhenChecked>
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==Disease==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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Known diseases associated with this structure: HIV-1 disease, delayed progression of OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=187011 187011]], HIV-1 disease, rapid progression of OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=187011 187011]]
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hrj ConSurf].
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1HRJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HRJ OCA].
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<div style="clear:both"></div>
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== References ==
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==Reference==
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<references/>
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The three-dimensional solution structure of RANTES., Chung CW, Cooke RM, Proudfoot AE, Wells TN, Biochemistry. 1995 Jul 25;34(29):9307-14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7542919 7542919]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chung, C.]]
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[[Category: Chung C]]
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[[Category: Cooke, R M.]]
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[[Category: Cooke RM]]
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[[Category: Proudfoot, A E.I.]]
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[[Category: Proudfoot AEI]]
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[[Category: Wells, T N.C.]]
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[[Category: Wells TNC]]
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[[Category: cc chemokine]]
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[[Category: cytokine (chemotactic)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:40:44 2008''
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Current revision

HUMAN RANTES, NMR, 13 STRUCTURES

PDB ID 1hrj

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