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| | ==TOG domain structure from C.elegans Zyg9== | | ==TOG domain structure from C.elegans Zyg9== |
| - | <StructureSection load='2of3' size='340' side='right' caption='[[2of3]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='2of3' size='340' side='right'caption='[[2of3]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2of3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OF3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OF3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2of3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OF3 FirstGlance]. <br> |
| - | </td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">zyg-9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2of3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2of3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2of3 RCSB], [http://www.ebi.ac.uk/pdbsum/2of3 PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2of3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2of3 OCA], [https://pdbe.org/2of3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2of3 RCSB], [https://www.ebi.ac.uk/pdbsum/2of3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2of3 ProSAT]</span></td></tr> |
| - | <table> | + | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/ZYG9_CAEEL ZYG9_CAEEL] Plays a major role in organizing microtubules and spindle poles during mitosis and meiosis in one-cell stage embryos (PubMed:16971515). Required for default nucleus positioning in oocytes (PubMed:16971515).<ref>PMID:12956952</ref> <ref>PMID:15866166</ref> <ref>PMID:16054029</ref> <ref>PMID:16971515</ref> <ref>PMID:17666432</ref> <ref>PMID:2249759</ref> <ref>PMID:3949074</ref> <ref>PMID:6684994</ref> <ref>PMID:9606208</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> | | Check<jmol> |
| | <jmolCheckbox> | | <jmolCheckbox> |
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/of/2of3_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/of/2of3_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
| - | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2of3 ConSurf]. |
| | <div style="clear:both"></div> | | <div style="clear:both"></div> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 2of3" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Caenorhabditis elegans]] | | [[Category: Caenorhabditis elegans]] |
| - | [[Category: Al-Bassam, J.]] | + | [[Category: Large Structures]] |
| - | [[Category: Harrison, S C.]] | + | [[Category: Al-Bassam J]] |
| - | [[Category: Hyman, A A.]] | + | [[Category: Harrison SC]] |
| - | [[Category: Larsen, N A.]] | + | [[Category: Hyman AA]] |
| - | [[Category: Cell cycle]]
| + | [[Category: Larsen NA]] |
| - | [[Category: Dis1]]
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| - | [[Category: Kinetochore]]
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| - | [[Category: Microtubule]]
| + | |
| - | [[Category: Microtubule associated protein]]
| + | |
| - | [[Category: Multifunctional macromolecule]]
| + | |
| - | [[Category: Structural protein]]
| + | |
| - | [[Category: Stu2]]
| + | |
| - | [[Category: Xmap215]]
| + | |
| - | [[Category: Zyg9]]
| + | |
| Structural highlights
Function
ZYG9_CAEEL Plays a major role in organizing microtubules and spindle poles during mitosis and meiosis in one-cell stage embryos (PubMed:16971515). Required for default nucleus positioning in oocytes (PubMed:16971515).[1] [2] [3] [4] [5] [6] [7] [8] [9]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Members of the XMAP215/Dis1 family of microtubule-associated proteins (MAPs) are essential for microtubule growth. MAPs in this family contain several 250 residue repeats, called TOG domains, which are thought to bind tubulin dimers and promote microtubule polymerization. We have determined the crystal structure of a single TOG domain from the Caenorhabditis elegans homolog, Zyg9, to 1.9 A resolution, and from it we describe a structural blueprint for TOG domains. These domains are flat, paddle-like structures, composed of six HEAT-repeat elements stacked side by side. The two wide faces of the paddle contain the HEAT-repeat helices, and the two narrow faces, the intra- and inter-HEAT repeat turns. Solvent-exposed residues in the intrarepeat turns are conserved, both within a particular protein and across the XMAP215/Dis1 family. Mutation of some of these residues in the TOG1 domain from the budding yeast homolog, Stu2p, shows that this face indeed participates in the tubulin contact.
Crystal structure of a TOG domain: conserved features of XMAP215/Dis1-family TOG domains and implications for tubulin binding.,Al-Bassam J, Larsen NA, Hyman AA, Harrison SC Structure. 2007 Mar;15(3):355-62. PMID:17355870[10]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Srayko M, Quintin S, Schwager A, Hyman AA. Caenorhabditis elegans TAC-1 and ZYG-9 form a complex that is essential for long astral and spindle microtubules. Curr Biol. 2003 Sep 2;13(17):1506-11. PMID:12956952 doi:10.1016/s0960-9822(03)00597-9
- ↑ Kimura A, Onami S. Computer simulations and image processing reveal length-dependent pulling force as the primary mechanism for C. elegans male pronuclear migration. Dev Cell. 2005 May;8(5):765-75. PMID:15866166 doi:10.1016/j.devcel.2005.03.007
- ↑ Srayko M, Kaya A, Stamford J, Hyman AA. Identification and characterization of factors required for microtubule growth and nucleation in the early C. elegans embryo. Dev Cell. 2005 Aug;9(2):223-36. PMID:16054029 doi:10.1016/j.devcel.2005.07.003
- ↑ DeBella LR, Hayashi A, Rose LS. LET-711, the Caenorhabditis elegans NOT1 ortholog, is required for spindle positioning and regulation of microtubule length in embryos. Mol Biol Cell. 2006 Nov;17(11):4911-24. PMID:16971515 doi:10.1091/mbc.e06-02-0107
- ↑ Bellanger JM, Carter JC, Phillips JB, Canard C, Bowerman B, Gönczy P. ZYG-9, TAC-1 and ZYG-8 together ensure correct microtubule function throughout the cell cycle of C. elegans embryos. J Cell Sci. 2007 Aug 15;120(Pt 16):2963-73. PMID:17666432 doi:10.1242/jcs.004812
- ↑ Mains PE, Kemphues KJ, Sprunger SA, Sulston IA, Wood WB. Mutations affecting the meiotic and mitotic divisions of the early Caenorhabditis elegans embryo. Genetics. 1990 Nov;126(3):593-605. PMID:2249759 doi:10.1093/genetics/126.3.593
- ↑ Kemphues KJ, Wolf N, Wood WB, Hirsh D. Two loci required for cytoplasmic organization in early embryos of Caenorhabditis elegans. Dev Biol. 1986 Feb;113(2):449-60. PMID:3949074 doi:10.1016/0012-1606(86)90180-6
- ↑ Strome S, Wood WB. Generation of asymmetry and segregation of germ-line granules in early C. elegans embryos. Cell. 1983 Nov;35(1):15-25. PMID:6684994 doi:10.1016/0092-8674(83)90203-9
- ↑ Matthews LR, Carter P, Thierry-Mieg D, Kemphues K. ZYG-9, a Caenorhabditis elegans protein required for microtubule organization and function, is a component of meiotic and mitotic spindle poles. J Cell Biol. 1998 Jun 1;141(5):1159-68. PMID:9606208 doi:10.1083/jcb.141.5.1159
- ↑ Al-Bassam J, Larsen NA, Hyman AA, Harrison SC. Crystal structure of a TOG domain: conserved features of XMAP215/Dis1-family TOG domains and implications for tubulin binding. Structure. 2007 Mar;15(3):355-62. PMID:17355870 doi:10.1016/j.str.2007.01.012
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