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| - | ==STRUCTURE OF THE PHAGE P2 BASEPLATE IN ITS ACTIVATED CONFORMATION WITH CA (PART 1 OF 2)==
| + | #REDIRECT [[4v5i]] This PDB entry is obsolete and replaced by 4v5i |
| - | <StructureSection load='2x54' size='340' side='right' caption='[[2x54]], [[Resolution|resolution]] 5.46Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[2x54]] is a 27 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_phage_p2 Lactococcus phage p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X54 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X54 FirstGlance]. <br>
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| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
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| - | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2x5a|2x5a]], [[2x53|2x53]], [[2wzp|2wzp]]</td></tr>
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| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x54 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x54 RCSB], [http://www.ebi.ac.uk/pdbsum/2x54 PDBsum]</span></td></tr>
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| - | <table>
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| - | == Evolutionary Conservation ==
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| - | [[Image:Consurf_key_small.gif|200px|right]]
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| - | Check<jmol>
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| - | <jmolCheckbox>
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| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x5/2x54_consurf.spt"</scriptWhenChecked>
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| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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| - | <text>to colour the structure by Evolutionary Conservation</text>
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| - | </jmolCheckbox>
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| - | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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| - | <div style="clear:both"></div>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Siphoviridae is the most abundant viral family on earth which infects bacteria as well as archaea. All known siphophages infecting gram+ Lactococcus lactis possess a baseplate at the tip of their tail involved in host recognition and attachment. Here, we report analysis of the p2 phage baseplate structure by X-ray crystallography and electron microscopy and propose a mechanism for the baseplate activation during attachment to the host cell. This approximately 1 MDa, Escherichia coli-expressed baseplate is composed of three protein species, including six trimers of the receptor-binding protein (RBP). RBPs host-recognition domains point upwards, towards the capsid, in agreement with the electron-microscopy map of the free virion. In the presence of Ca(2+), a cation mandatory for infection, the RBPs rotated 200 degrees downwards, presenting their binding sites to the host, and a channel opens at the bottom of the baseplate for DNA passage. These conformational changes reveal a novel siphophage activation and host-recognition mechanism leading ultimately to DNA ejection.
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| - | Structure of lactococcal phage p2 baseplate and its mechanism of activation.,Sciara G, Bebeacua C, Bron P, Tremblay D, Ortiz-Lombardia M, Lichiere J, van Heel M, Campanacci V, Moineau S, Cambillau C Proc Natl Acad Sci U S A. 2010 Mar 29. PMID:20351260<ref>PMID:20351260</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Lactococcus phage p2]]
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| - | [[Category: Bebeacua, C.]]
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| - | [[Category: Bron, P.]]
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| - | [[Category: Cambillau, C.]]
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| - | [[Category: Campanacci, V.]]
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| - | [[Category: Lichiere, J.]]
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| - | [[Category: Moineau, S.]]
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| - | [[Category: Ortiz-Lombardia, M.]]
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| - | [[Category: Sciara, G.]]
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| - | [[Category: Tremblay, D.]]
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| - | [[Category: Vanheel, M.]]
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| - | [[Category: Lactococcus lacti]]
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| - | [[Category: Siphoviridae]]
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| - | [[Category: Viral protein]]
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