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2ks6

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==NMR solution structure of ALG13 --- obtained with iterative CS-Rosetta from backbone NMR data.==
==NMR solution structure of ALG13 --- obtained with iterative CS-Rosetta from backbone NMR data.==
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<StructureSection load='2ks6' size='340' side='right' caption='[[2ks6]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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<StructureSection load='2ks6' size='340' side='right'caption='[[2ks6]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2ks6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KS6 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2ks6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KS6 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2jzc|2jzc]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ALG13, YGL047W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ks6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ks6 OCA], [https://pdbe.org/2ks6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ks6 RCSB], [https://www.ebi.ac.uk/pdbsum/2ks6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ks6 ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylglucosaminyldiphosphodolichol_N-acetylglucosaminyltransferase N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.141 2.4.1.141] </span></td></tr>
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</table>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ks6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ks6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ks6 RCSB], [http://www.ebi.ac.uk/pdbsum/2ks6 PDBsum]</span></td></tr>
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== Function ==
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<table>
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[https://www.uniprot.org/uniprot/ALG13_YEAST ALG13_YEAST] Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway.<ref>PMID:15615718</ref> <ref>PMID:16100110</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Conventional protein structure determination from nuclear magnetic resonance data relies heavily on side-chain proton-to-proton distances. The necessary side-chain resonance assignment, however, is labor intensive and prone to error. Here we show that structures can be accurately determined without nuclear magnetic resonance (NMR) information on the side chains for proteins up to 25 kilodaltons by incorporating backbone chemical shifts, residual dipolar couplings, and amide proton distances into the Rosetta protein structure modeling methodology. These data, which are too sparse for conventional methods, serve only to guide conformational search toward the lowest-energy conformations in the folding landscape; the details of the computed models are determined by the physical chemistry implicit in the Rosetta all-atom energy function. The new method is not hindered by the deuteration required to suppress nuclear relaxation processes for proteins greater than 15 kilodaltons and should enable routine NMR structure determination for larger proteins.
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NMR structure determination for larger proteins using backbone-only data.,Raman S, Lange OF, Rossi P, Tyka M, Wang X, Aramini J, Liu G, Ramelot TA, Eletsky A, Szyperski T, Kennedy MA, Prestegard J, Montelione GT, Baker D Science. 2010 Feb 19;327(5968):1014-8. Epub 2010 Feb 4. PMID:20133520<ref>PMID:20133520</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Atcc 18824]]
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[[Category: Large Structures]]
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[[Category: N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Baker, D.]]
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[[Category: Baker D]]
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[[Category: Lange, O F.]]
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[[Category: Lange OF]]
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[[Category: Prestegard, J H.]]
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[[Category: Prestegard JH]]
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[[Category: Wang, X.]]
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[[Category: Wang X]]
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[[Category: Cs-rosetta]]
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[[Category: Cyclic n-oxide]]
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[[Category: Endoplasmic reticulum]]
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[[Category: Glycosyltransferase]]
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[[Category: Transferase]]
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Current revision

NMR solution structure of ALG13 --- obtained with iterative CS-Rosetta from backbone NMR data.

PDB ID 2ks6

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