4wis
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the lipid scramblase nhTMEM16 in crystal form 1== | |
| + | <StructureSection load='4wis' size='340' side='right'caption='[[4wis]], [[Resolution|resolution]] 3.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4wis]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_vanettenii_77-13-4 Fusarium vanettenii 77-13-4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WIS FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wis OCA], [https://pdbe.org/4wis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wis RCSB], [https://www.ebi.ac.uk/pdbsum/4wis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wis ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/C7Z7K1_FUSV7 C7Z7K1_FUSV7] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The TMEM16 family of proteins, also known as anoctamins, features a remarkable functional diversity. This family contains the long sought-after Ca2+-activated chloride channels as well as lipid scramblases and cation channels. Here we present the crystal structure of a TMEM16 family member from the fungus Nectria haematococca that operates as a Ca2+-activated lipid scramblase. Each subunit of the homodimeric protein contains ten transmembrane helices and a hydrophilic membrane-traversing cavity that is exposed to the lipid bilayer as a potential site of catalysis. This cavity harbours a conserved Ca2+-binding site located within the hydrophobic core of the membrane. Mutations of residues involved in Ca2+ coordination affect both lipid scrambling in N. haematococca TMEM16 and ion conduction in the Cl- channel TMEM16A. The structure reveals the general architecture of the family and its mode of Ca2+ activation. It also provides insight into potential scrambling mechanisms and serves as a framework to unravel the conduction of ions in certain TMEM16 proteins. | ||
| - | + | X-ray structure of a calcium-activated TMEM16 lipid scramblase.,Brunner JD, Lim NK, Schenck S, Duerst A, Dutzler R Nature. 2014 Nov 12. doi: 10.1038/nature13984. PMID:25383531<ref>PMID:25383531</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4wis" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Anoctamin 3D structures|Anoctamin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Fusarium vanettenii 77-13-4]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Brunner JD]] | ||
| + | [[Category: Dutzler R]] | ||
| + | [[Category: Lim NK]] | ||
| + | [[Category: Schenck S]] | ||
Current revision
Crystal structure of the lipid scramblase nhTMEM16 in crystal form 1
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