2wkc

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure from a single-stranded DNA binding protein from the lactococcal phage p2

Structural highlights

2wkc is a 4 chain structure with sequence from Lactococcus virus P2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.6Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q09WL7_9CAUD

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Lactococcus lactis, a Gram-positive bacterium widely used by the dairy industry, is subject to infection by a diverse population of virulent phages, predominantly by those of the 936 group, including the siphovirus phage p2. Confronted with the negative impact of phage infection on milk fermentation, the study of the biology of lactococcal provides insight from applied and fundamental perspectives. We decided to characterize the product of the orf34 gene from lactococcus phage p2, which was considered as a candidate single-stranded DNA binding protein (SSB) due to its localization downstream of a gene coding for a single-strand annealing protein. Two-dimensional gel electrophoresis showed that ORF34(p2) is expressed in large amounts during the early phases of phage infection, suggesting an important role in this process. Gel-shift assays, surface plasmon resonance and atomic force microscopy demonstrated that ORF34(p2) interacts with single-strand DNA with nanomolar affinity. We also determined the crystal structure of ORF34(p2) and showed that it bears a variation of the typical oligonucleotide/oligosaccharide binding-fold of SSBs. Finally, we found that ORF34(p2) is able to stimulate Escherichia coli RecA-mediated homologous recombination. The specific structural and biochemical properties that distinguish ORF34(p2) from other SSB proteins are discussed.

Structure and function of phage p2 ORF34(p2), a new type of single-stranded DNA binding protein.,Scaltriti E, Tegoni M, Rivetti C, Launay H, Masson JY, Magadan AH, Tremblay D, Moineau S, Ramoni R, Lichiere J, Campanacci V, Cambillau C, Ortiz-Lombardia M Mol Microbiol. 2009 Sep;73(6):1156-70. Epub 2009 Aug 25. PMID:19719513^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Scaltriti E, Tegoni M, Rivetti C, Launay H, Masson JY, Magadan AH, Tremblay D, Moineau S, Ramoni R, Lichiere J, Campanacci V, Cambillau C, Ortiz-Lombardia M. Structure and function of phage p2 ORF34(p2), a new type of single-stranded DNA binding protein. Mol Microbiol. 2009 Sep;73(6):1156-70. Epub 2009 Aug 25. PMID:19719513 doi:10.1111/j.1365-2958.2009.06844.x

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wkc"

Categories: Lactococcus virus P2 | Large Structures | Cambillau C | Ortiz-Lombardia M | Scaltriti E

@@ Line 1: / Line 1: @@
-==CRYSTAL STRUCTURE FROM A SINGLE-STRANDED DNA BINDING PROTEIN FROM THE LACTOCOCCAL PHAGE P2==
-<StructureSection load='2wkc' size='340' side='right' caption='[[2wkc]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+==Crystal structure from a single-stranded DNA binding protein from the lactococcal phage p2==
+<StructureSection load='2wkc' size='340' side='right'caption='[[2wkc]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2wkc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_phage_p2 Lactococcus phage p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WKC FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2wkc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_virus_P2 Lactococcus virus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WKC FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wkd|2wkd]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wkc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wkc RCSB], [http://www.ebi.ac.uk/pdbsum/2wkc PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wkc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkc OCA], [https://pdbe.org/2wkc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wkc RCSB], [https://www.ebi.ac.uk/pdbsum/2wkc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wkc ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q09WL7_9CAUD Q09WL7_9CAUD]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wk/2wkc_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wk/2wkc_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wkc ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 24: / Line 27: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2wkc" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Lactococcus phage p2]]
+[[Category: Lactococcus virus P2]]
-[[Category: Cambillau, C.]]
+[[Category: Large Structures]]
-[[Category: Ortiz-Lombardia, M.]]
+[[Category: Cambillau C]]
-[[Category: Scaltriti, E.]]
+[[Category: Ortiz-Lombardia M]]
-[[Category: Dna binding protein]]
+[[Category: Scaltriti E]]
-[[Category: Lactococcal phage protein]]
-[[Category: Single-stranded dna binding]]
-[[Category: Ssb]]

2wkc

From Proteopedia

Current revision

Crystal structure from a single-stranded DNA binding protein from the lactococcal phage p2

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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