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3j8c

From Proteopedia

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Current revision

Model of the human eIF3 PCI-MPN octamer docked into the 43S EM map

3j8c, resolution 11.60Å

Structural highlights

3j8c is a 8 chain structure with sequence from Homo sapiens. This structure supersedes the now removed PDB entry 3j7k. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 11.6Å
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

EIF3A_HUMAN RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815).[HAMAP-Rule:MF_03000]^[1] ^[2] ^[3] ^[4] (Microbial infection) Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634).^[5] ^[6] (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).^[7]

References

↑ Lin L, Holbro T, Alonso G, Gerosa D, Burger MM. Molecular interaction between human tumor marker protein p150, the largest subunit of eIF3, and intermediate filament protein K7. J Cell Biochem. 2001;80(4):483-90. PMID:11169732 doi:<483::aid-jcb1002>3.0.co;2-b 10.1002/1097-4644(20010315)80:4<483::aid-jcb1002>3.0.co;2-b
↑ Masutani M, Sonenberg N, Yokoyama S, Imataka H. Reconstitution reveals the functional core of mammalian eIF3. EMBO J. 2007 Jul 25;26(14):3373-83. doi: 10.1038/sj.emboj.7601765. Epub 2007 Jun , 21. PMID:17581632 doi:http://dx.doi.org/10.1038/sj.emboj.7601765
↑ Lee AS, Kranzusch PJ, Cate JH. eIF3 targets cell-proliferation messenger RNAs for translational activation or repression. Nature. 2015 Jun 4;522(7554):111-4. doi: 10.1038/nature14267. Epub 2015 Apr 6. PMID:25849773 doi:http://dx.doi.org/10.1038/nature14267
↑ Lee AS, Kranzusch PJ, Doudna JA, Cate JH. eIF3d is an mRNA cap-binding protein that is required for specialized translation initiation. Nature. 2016 Aug 4;536(7614):96-9. PMID:27462815 doi:http://dx.doi.org/10.1038/nature18954
↑ Sun C, Querol-Audí J, Mortimer SA, Arias-Palomo E, Doudna JA, Nogales E, Cate JH. Two RNA-binding motifs in eIF3 direct HCV IRES-dependent translation. Nucleic Acids Res. 2013 Aug;41(15):7512-21. PMID:23766293 doi:10.1093/nar/gkt510
↑ Sweeney TR, Abaeva IS, Pestova TV, Hellen CU. The mechanism of translation initiation on Type 1 picornavirus IRESs. EMBO J. 2014 Jan 7;33(1):76-92. PMID:24357634 doi:10.1002/embj.201386124
↑ Poyry TA, Kaminski A, Connell EJ, Fraser CS, Jackson RJ. The mechanism of an exceptional case of reinitiation after translation of a long ORF reveals why such events do not generally occur in mammalian mRNA translation. Genes Dev. 2007 Dec 1;21(23):3149-62. doi: 10.1101/gad.439507. PMID:18056426 doi:http://dx.doi.org/10.1101/gad.439507

1 Structural highlights
2 Function
3 See Also
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j8c"

Categories: Homo sapiens | Large Structures | Ban N | Erzberger JP

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 3j8c is ON HOLD
+==Model of the human eIF3 PCI-MPN octamer docked into the 43S EM map==
+<SX load='3j8c' size='340' side='right' viewer='molstar' caption='[[3j8c]], [[Resolution|resolution]] 11.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3j8c]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3j7k 3j7k]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J8C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J8C FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 11.6&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j8c OCA], [https://pdbe.org/3j8c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j8c RCSB], [https://www.ebi.ac.uk/pdbsum/3j8c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j8c ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/EIF3A_HUMAN EIF3A_HUMAN] RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815).[HAMAP-Rule:MF_03000]<ref>PMID:11169732</ref> <ref>PMID:17581632</ref> <ref>PMID:25849773</ref> <ref>PMID:27462815</ref>   (Microbial infection) Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634).<ref>PMID:23766293</ref> <ref>PMID:24357634</ref>   (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).<ref>PMID:18056426</ref>
-Authors: Erzberger, J.P., Ban, N.
+==See Also==
+*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
-Description: Model of the human eIF3 PCI-MPN octamer docked into the 43S EM map
+== References ==
+<references/>
+__TOC__
+</SX>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Ban N]]
+[[Category: Erzberger JP]]

3j8c

From Proteopedia

Current revision

Model of the human eIF3 PCI-MPN octamer docked into the 43S EM map

Structural highlights

Function

See Also

References

Contents

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