4wl1
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4wl1 is ON HOLD Authors: Kalynych S, Cherney M, Cygler M Description: WzzE Polysaccharide Co-polymerase) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of WzzE Polysaccharide Co-polymerase== | |
| + | <StructureSection load='4wl1' size='340' side='right'caption='[[4wl1]], [[Resolution|resolution]] 5.99Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4wl1]] is a 32 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WL1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 5.989Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wl1 OCA], [https://pdbe.org/4wl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wl1 RCSB], [https://www.ebi.ac.uk/pdbsum/4wl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wl1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/WZZE_ECO57 WZZE_ECO57] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacteria have evolved cellular control mechanisms to ensure proper length specification for surface-bound polysaccharides. Members of the Polysaccharide Co-Polymerase (PCP) family are central to this process. PCP-1 family members are anchored to the inner membrane through two transmembrane helices and contain a large periplasm-exposed domain. PCPs are known to form homooligomers but their exact stoichiometry is controversial in view of conflicting structural and biochemical data. Several prior investigations addressing this question indicated a nonameric, hexameric or tetrameric organization of several PCP-1 family members. In this work, we gathered additional evidence that E.coli WzzB and WzzE PCPs form octameric homo-oligomeric complexes. Detergent-solubilized PCPs were purified to homogeneity and subjected to blue native gel analysis, which indicated the presence of a predominant high-molecular product of over 500 kDa in mass. Molecular mass of WzzE and WzzB-detergent oligomers was estimated to be 550 kDA by size-exclusion coupled to multi-angle laser light scattering (SEC-MALLS). Oligomeric organization of purified WzzB and WzzE was further investigated by negative stain electron microscopy and by X-ray crystallography, respectively. Analysis of EM-derived molecular envelope of WzzB indicated that the full-length protein is composed of eight protomers. Crystal structure of LDAO-solubilized WzzE was solved to 6A resolution and revealed its octameric subunit stoichiometry. In summary, we identified a possible biological unit utilized for the glycan chain length determination by two PCP-1 family members. This provides an important step toward further unraveling of the mechanistic basis of chain length control of the O-antigen and the enterobacterial common antigen. | ||
| - | + | Quaternary structure of WzzB and WzzE polysaccharide co-polymerases.,Kalynych S, Cherney M, Bostina M, Rouiller I, Cygler M Protein Sci. 2014 Oct 11. doi: 10.1002/pro.2586. PMID:25307743<ref>PMID:25307743</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4wl1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Escherichia coli O157:H7]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Cherney M]] | ||
| + | [[Category: Cygler M]] | ||
| + | [[Category: Kalynych S]] | ||
Current revision
Structure of WzzE Polysaccharide Co-polymerase
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