1k68

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (22:05, 26 March 2025) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1k68.jpg|left|200px]]
 
-
{{Structure
+
==Crystal Structure of the Phosphorylated Cyanobacterial Phytochrome Response Regulator RcpA==
-
|PDB= 1k68 |SIZE=350|CAPTION= <scene name='initialview01'>1k68</scene>, resolution 1.90&Aring;
+
<StructureSection load='1k68' size='340' side='right'caption='[[1k68]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
+
<table><tr><td colspan='2'>[[1k68]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tolypothrix_sp._PCC_7601 Tolypothrix sp. PCC 7601]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K68 FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-
|GENE= AF309559 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1188 Tolypothrix sp. PCC 7601])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PHD:ASPARTYL+PHOSPHATE'>PHD</scene></td></tr>
-
}}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k68 OCA], [https://pdbe.org/1k68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k68 RCSB], [https://www.ebi.ac.uk/pdbsum/1k68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k68 ProSAT]</span></td></tr>
-
 
+
</table>
-
'''Crystal Structure of the Phosphorylated Cyanobacterial Phytochrome Response Regulator RcpA'''
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/Q8RTN0_9CYAN Q8RTN0_9CYAN]
-
 
+
== Evolutionary Conservation ==
-
==Overview==
+
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k6/1k68_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k68 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
The structures of two response regulators (RRs) from the cyanobacterium Calothrix PCC7601, RcpA and RcpB, were solved to 1.9- and 1.75-A resolution, respectively. RcpA was found in phosphorylated and RcpB in nonphosphorylated form. Both RRs are members of phytochrome-associated, light-sensing two-component signal transduction pathways, based on histidine kinase-mediated receptor autophosphorylation and phosphorelay to a RR. Despite the overall folding similarity to CheY-type RRs ((beta/alpha)(5)-motif), RcpA and RcpB form homodimers, irrespective of their phosphorylation state, giving insight into a signal transduction putatively different from that of other known RRs. Dimerization is accomplished by a C-terminal extension of the RR polypeptide chain, and the surface formed by H4, beta 5, and H5, which constitute a hydrophobic contact area with distinct interactions between residues of either subunit. Sequence alignments reveal that the identified dimerization motif is archetypal for phytochrome-associated RRs, making them a novel subgroup of CheY-type RRs. The protein structures of RcpA and RcpB are compared to the recently presented protein structure of Rcp1 from Synechocystis.
The structures of two response regulators (RRs) from the cyanobacterium Calothrix PCC7601, RcpA and RcpB, were solved to 1.9- and 1.75-A resolution, respectively. RcpA was found in phosphorylated and RcpB in nonphosphorylated form. Both RRs are members of phytochrome-associated, light-sensing two-component signal transduction pathways, based on histidine kinase-mediated receptor autophosphorylation and phosphorelay to a RR. Despite the overall folding similarity to CheY-type RRs ((beta/alpha)(5)-motif), RcpA and RcpB form homodimers, irrespective of their phosphorylation state, giving insight into a signal transduction putatively different from that of other known RRs. Dimerization is accomplished by a C-terminal extension of the RR polypeptide chain, and the surface formed by H4, beta 5, and H5, which constitute a hydrophobic contact area with distinct interactions between residues of either subunit. Sequence alignments reveal that the identified dimerization motif is archetypal for phytochrome-associated RRs, making them a novel subgroup of CheY-type RRs. The protein structures of RcpA and RcpB are compared to the recently presented protein structure of Rcp1 from Synechocystis.
-
==About this Structure==
+
Crystal structures of two cyanobacterial response regulators in apo- and phosphorylated form reveal a novel dimerization motif of phytochrome-associated response regulators.,Benda C, Scheufler C, Tandeau de Marsac N, Gartner W Biophys J. 2004 Jul;87(1):476-87. PMID:15240481<ref>PMID:15240481</ref>
-
1K68 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Tolypothrix_sp._pcc_7601 Tolypothrix sp. pcc 7601]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K68 OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Crystal structures of two cyanobacterial response regulators in apo- and phosphorylated form reveal a novel dimerization motif of phytochrome-associated response regulators., Benda C, Scheufler C, Tandeau de Marsac N, Gartner W, Biophys J. 2004 Jul;87(1):476-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15240481 15240481]
+
</div>
-
[[Category: Single protein]]
+
<div class="pdbe-citations 1k68" style="background-color:#fffaf0;"></div>
-
[[Category: Tolypothrix sp. pcc 7601]]
+
-
[[Category: Benda, C.]]
+
-
[[Category: Gaertner, W.]]
+
-
[[Category: Marsac, N Tandeau de.]]
+
-
[[Category: Scheufler, C.]]
+
-
[[Category: MG]]
+
-
[[Category: (beta/alpha)5]]
+
-
[[Category: chey homologue]]
+
-
[[Category: homodimer]]
+
-
[[Category: phosphorylated aspartate]]
+
-
[[Category: response regulator]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:13:08 2008''
+
==See Also==
 +
*[[Response regulator 3D structure|Response regulator 3D structure]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Tolypothrix sp. PCC 7601]]
 +
[[Category: Benda C]]
 +
[[Category: Gaertner W]]
 +
[[Category: Scheufler C]]
 +
[[Category: Tandeau de Marsac N]]

Current revision

Crystal Structure of the Phosphorylated Cyanobacterial Phytochrome Response Regulator RcpA

PDB ID 1k68

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1k68"
Personal tools