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| | ==An auxilin-like J-domain containing protein, JAC1 J-domain== | | ==An auxilin-like J-domain containing protein, JAC1 J-domain== |
| - | <StructureSection load='3ag7' size='340' side='right' caption='[[3ag7]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='3ag7' size='340' side='right'caption='[[3ag7]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ag7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AG7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AG7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ag7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AG7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AG7 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1nz6|1nz6]], [[2qwo|2qwo]], [[2qwr|2qwr]], [[2qwp|2qwp]], [[2qwq|2qwq]], [[2qwn|2qwn]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">At1g75100, F9E10.5, JAC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ag7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ag7 OCA], [https://pdbe.org/3ag7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ag7 RCSB], [https://www.ebi.ac.uk/pdbsum/3ag7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ag7 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ag7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ag7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ag7 RCSB], [http://www.ebi.ac.uk/pdbsum/3ag7 PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/JAC1_ARATH JAC1_ARATH] Required for chloroplast photorelocation movement; chloroplast accumulation upon low blue light and for chloroplast movement to the bottom of cells in darkness, by modulating chloroplast actin (Cp-actin) filaments distribution, appearance and disappearance. May mediate a slight resistance to aluminum in root hair cells.<ref>PMID:16113208</ref> <ref>PMID:17150990</ref> <ref>PMID:20562448</ref> <ref>PMID:21737483</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 3ag7" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Arabidopsis thaliana]] | | [[Category: Arabidopsis thaliana]] |
| - | [[Category: Takano, A.]] | + | [[Category: Large Structures]] |
| - | [[Category: An auxilin-like j-domain containing protein]] | + | [[Category: Takano A]] |
| - | [[Category: Chloroplast accumulation response]]
| + | |
| - | [[Category: J-domain]]
| + | |
| - | [[Category: Jac1]]
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| - | [[Category: Plant protein]]
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| Structural highlights
Function
JAC1_ARATH Required for chloroplast photorelocation movement; chloroplast accumulation upon low blue light and for chloroplast movement to the bottom of cells in darkness, by modulating chloroplast actin (Cp-actin) filaments distribution, appearance and disappearance. May mediate a slight resistance to aluminum in root hair cells.[1] [2] [3] [4]
Publication Abstract from PubMed
An auxilin-like J-domain-containing protein, JAC1, is necessary for chloroplast movement in Arabidopsis thaliana, to capture photosynthetic light efficiently under weak light conditions. Here, we performed crystallographic and functional analyses of the J-domain of JAC1. The crystal structure of the J-domain is quite similar to that of bovine auxilin, and possesses a similar positively charged surface, which probably forms the interface with the Hsp70 chaperone. The mutation of the highly conserved HPD motif of the JAC1 J-domain abrogated the chloroplast photorelocation response. These results suggest that the requirement of JAC1 in chloroplast photorelocation movement is attributable to the J-domain's cochaperone activity.
Crystallographic and functional analyses of J-domain of JAC1 essential for chloroplast photorelocation movement in Arabidopsis thaliana.,Takano A, Suetsugu N, Wada M, Kohda D Plant Cell Physiol. 2010 Aug;51(8):1372-6. Epub 2010 Jun 20. PMID:20562448[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Suetsugu N, Kagawa T, Wada M. An auxilin-like J-domain protein, JAC1, regulates phototropin-mediated chloroplast movement in Arabidopsis. Plant Physiol. 2005 Sep;139(1):151-62. Epub 2005 Aug 19. PMID:16113208 doi:http://dx.doi.org/10.1104/pp.105.067371
- ↑ Ezaki B, Kiyohara H, Matsumoto H, Nakashima S. Overexpression of an auxilin-like gene (F9E10.5) can suppress Al uptake in roots of Arabidopsis. J Exp Bot. 2007;58(3):497-506. Epub 2006 Dec 5. PMID:17150990 doi:http://dx.doi.org/10.1093/jxb/erl221
- ↑ Takano A, Suetsugu N, Wada M, Kohda D. Crystallographic and functional analyses of J-domain of JAC1 essential for chloroplast photorelocation movement in Arabidopsis thaliana. Plant Cell Physiol. 2010 Aug;51(8):1372-6. Epub 2010 Jun 20. PMID:20562448 doi:10.1093/pcp/pcq089
- ↑ Ichikawa S, Yamada N, Suetsugu N, Wada M, Kadota A. Red light, Phot1 and JAC1 modulate Phot2-dependent reorganization of chloroplast actin filaments and chloroplast avoidance movement. Plant Cell Physiol. 2011 Aug;52(8):1422-32. doi: 10.1093/pcp/pcr087. Epub 2011, Jul 7. PMID:21737483 doi:http://dx.doi.org/10.1093/pcp/pcr087
- ↑ Takano A, Suetsugu N, Wada M, Kohda D. Crystallographic and functional analyses of J-domain of JAC1 essential for chloroplast photorelocation movement in Arabidopsis thaliana. Plant Cell Physiol. 2010 Aug;51(8):1372-6. Epub 2010 Jun 20. PMID:20562448 doi:10.1093/pcp/pcq089
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