4d48
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4d48 is ON HOLD Authors: Toccafondi, M., Wuerges, J., Cianci, M., Benini, S. Description: Crystal Structure of glucose-1-phosphate uridylyltransfer...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of glucose-1-phosphate uridylyltransferase GalU from Erwinia amylovora.== | |
| + | <StructureSection load='4d48' size='340' side='right'caption='[[4d48]], [[Resolution|resolution]] 2.46Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4d48]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Erwinia_amylovora_CFBP1430 Erwinia amylovora CFBP1430]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D48 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D48 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.46Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d48 OCA], [https://pdbe.org/4d48 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d48 RCSB], [https://www.ebi.ac.uk/pdbsum/4d48 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d48 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/D4I3X5_ERWAC D4I3X5_ERWAC] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Erwinia amylovora, a Gram-negative plant pathogen, is the causal agent of Fire Blight, a contagious necrotic disease affecting plants belonging to the Rosaceae family, including apple and pear. E. amylovora is highly virulent and capable of rapid dissemination in orchards; effective control methods are still lacking. One of its most important pathogenicity factors is the exopolysaccharide amylovoran. Amylovoran is a branched polymer made by the repetition of units mainly composed of galactose, with some residues of glucose, glucuronic acid and pyruvate. E. amylovora glucose-1-phosphate uridylyltransferase (UDP-glucose pyrophosphorylase, EC 2.7.7.9) has a key role in amylovoran biosynthesis. This enzyme catalyses the production of UDP-glucose from glucose-1-phosphate and UTP, which the epimerase GalE converts into UDP-galactose, the main building block of amylovoran. We determined EaGalU kinetic parameters and substrate specificity with a range of sugar 1-phosphates. At time point 120min the enzyme catalysed conversion of the sugar 1-phosphate into the corresponding UDP-sugar reached 74% for N-acetyl-alpha-d-glucosamine 1-phosphate, 28% for alpha-d-galactose 1-phosphate, 0% for alpha-d-galactosamine 1-phosphate, 100% for alpha-d-xylose 1-phosphate, 100% for alpha-d-glucosamine 1-phosphate, 70% for alpha-d-mannose 1-phosphate, and 0% for alpha-d-galacturonic acid 1-phosphate. To explain our results we obtained the crystal structure of EaGalU and augmented our study by docking the different sugar 1-phosphates into EaGalU active site, providing both reliable models for substrate binding and enzyme specificity, and a rationale that explains the different activity of EaGalU on the sugar 1-phosphates used. These data demonstrate EaGalU potential as a biocatalyst for biotechnological purposes, as an alternative to the enzyme from Escherichia coli, besides playing an important role in E. amylovora pathogenicity. | ||
| - | + | Glucose-1-phosphate uridylyltransferase from Erwinia amylovora: Activity, structure and substrate specificity.,Benini S, Toccafondi M, Rejzek M, Musiani F, Wagstaff BA, Wuerges J, Cianci M, Field RA Biochim Biophys Acta. 2017 Nov;1865(11 Pt A):1348-1357. doi:, 10.1016/j.bbapap.2017.08.015. Epub 2017 Aug 24. PMID:28844747<ref>PMID:28844747</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4d48" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Erwinia amylovora CFBP1430]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Benini S]] | ||
| + | [[Category: Cianci M]] | ||
| + | [[Category: Toccafondi M]] | ||
| + | [[Category: Wuerges J]] | ||
Current revision
Crystal Structure of glucose-1-phosphate uridylyltransferase GalU from Erwinia amylovora.
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