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| | ==The pseudo-atomic structure of dengue immature virus== | | ==The pseudo-atomic structure of dengue immature virus== |
| - | <StructureSection load='3c6d' size='340' side='right' caption='[[3c6d]], [[Resolution|resolution]] 12.50Å' scene=''> | + | <SX load='3c6d' size='340' side='right' viewer='molstar' caption='[[3c6d]], [[Resolution|resolution]] 12.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3c6d]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2] and [http://en.wikipedia.org/wiki/Dengue_virus_2_thailand/16681/84 Dengue virus 2 thailand/16681/84]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C6D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C6D FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3c6d]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2] and [https://en.wikipedia.org/wiki/Dengue_virus_2_Thailand/16681/84 Dengue virus 2 Thailand/16681/84]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C6D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C6D FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3c5x|3c5x]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12.5Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c6d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c6d RCSB], [http://www.ebi.ac.uk/pdbsum/3c6d PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c6d OCA], [https://pdbe.org/3c6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c6d RCSB], [https://www.ebi.ac.uk/pdbsum/3c6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c6d ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A7TUD3_9FLAV A7TUD3_9FLAV] Component of the viral RNA replication complex that functions in virion assembly and antagonizes the host immune response.[ARBA:ARBA00024317] Functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter.[ARBA:ARBA00003504] Serine protease subunit NS2B: Required cofactor for the serine protease function of NS3.[PROSITE-ProRule:PRU00859] |
| - | Many viruses go through a maturation step in the final stages of assembly before being transmitted to another host. The maturation process of flaviviruses is directed by the proteolytic cleavage of the precursor membrane protein (prM), turning inert virus into infectious particles. We have determined the 2.2 angstrom resolution crystal structure of a recombinant protein in which the dengue virus prM is linked to the envelope glycoprotein E. The structure represents the prM-E heterodimer and fits well into the cryo-electron microscopy density of immature virus at neutral pH. The pr peptide beta-barrel structure covers the fusion loop in E, preventing fusion with host cell membranes. The structure provides a basis for identifying the stages of its pH-directed conformational metamorphosis during maturation, ending with release of pr when budding from the host.
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| - | The flavivirus precursor membrane-envelope protein complex: structure and maturation.,Li L, Lok SM, Yu IM, Zhang Y, Kuhn RJ, Chen J, Rossmann MG Science. 2008 Mar 28;319(5871):1830-4. PMID:18369147<ref>PMID:18369147</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| | [[Category: Dengue virus 2]] | | [[Category: Dengue virus 2]] |
| - | [[Category: Dengue virus 2 thailand/16681/84]] | + | [[Category: Dengue virus 2 Thailand/16681/84]] |
| - | [[Category: Li, L.]] | + | [[Category: Large Structures]] |
| - | [[Category: Atp-binding]] | + | [[Category: Li L]] |
| - | [[Category: Capsid protein]]
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| - | [[Category: Cleavage on pair of basic residue]]
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| - | [[Category: Endoplasmic reticulum]]
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| - | [[Category: Envelope protein]]
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| - | [[Category: Glycoprotein]]
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| - | [[Category: Helicase]]
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| - | [[Category: Hydrolase]]
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| - | [[Category: Icosahedral virion]]
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| - | [[Category: Icosahedral virus]]
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| - | [[Category: Metal-binding]]
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| - | [[Category: Multifunctional enzyme]]
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| - | [[Category: Nucleotide-binding]]
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| - | [[Category: Nucleotidyltransferase]]
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| - | [[Category: Nucleus]]
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| - | [[Category: Phosphoprotein]]
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| - | [[Category: Protease]]
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| - | [[Category: Ribonucleoprotein]]
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| - | [[Category: Rna replication]]
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| - | [[Category: Rna-binding]]
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| - | [[Category: Rna-directed rna polymerase]]
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| - | [[Category: Secreted]]
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| - | [[Category: Serine protease]]
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| - | [[Category: Transcription]]
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| - | [[Category: Transcription regulation]]
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| - | [[Category: Transferase]]
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| - | [[Category: Transmembrane]]
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| - | [[Category: Viral nucleoprotein]]
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| - | [[Category: Virus]]
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