3cwf

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==Crystal structure of PAS domain of two-component sensor histidine kinase==
==Crystal structure of PAS domain of two-component sensor histidine kinase==
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<StructureSection load='3cwf' size='340' side='right' caption='[[3cwf]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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<StructureSection load='3cwf' size='340' side='right'caption='[[3cwf]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3cwf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CWF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CWF FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3cwf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CWF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CWF FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">phoR, BSU29100 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=135461 Bacillus subtilis subsp. subtilis])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cwf OCA], [https://pdbe.org/3cwf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cwf RCSB], [https://www.ebi.ac.uk/pdbsum/3cwf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cwf ProSAT], [https://www.topsan.org/Proteins/MCSG/3cwf TOPSAN]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cwf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cwf RCSB], [http://www.ebi.ac.uk/pdbsum/3cwf PDBsum], [http://www.topsan.org/Proteins/MCSG/3cwf TOPSAN]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/PHOR_BACSU PHOR_BACSU] Member of the two-component regulatory system PhoP/PhoR involved in the alkaline phosphatase genes regulation. PhoR may function as a membrane-associated protein kinase that phosphorylates PhoP in response to environmental signals.
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We present the crystal structure of the extracytoplasmic domain of the Bacillus subtilis PhoR sensor histidine kinase, part of a two-component system involved in adaptation to low environmental phosphate concentrations. In addition to the PhoR structure, we predict that the majority of the extracytoplasmic domains of B. subtilis sensor kinases will adopt a fold similar to the ubiquitous PAS domain.
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Extracytoplasmic PAS-like domains are common in signal transduction proteins.,Chang C, Tesar C, Gu M, Babnigg G, Joachimiak A, Pokkuluri PR, Szurmant H, Schiffer M J Bacteriol. 2010 Feb;192(4):1156-9. Epub 2009 Dec 11. PMID:20008068<ref>PMID:20008068</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacillus subtilis subsp. subtilis]]
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[[Category: Bacillus subtilis subsp. subtilis str. 168]]
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[[Category: Histidine kinase]]
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[[Category: Large Structures]]
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[[Category: Chang, C.]]
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[[Category: Chang C]]
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[[Category: Gu, M.]]
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[[Category: Gu M]]
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[[Category: Joachimiak, A.]]
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[[Category: Joachimiak A]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Tesar C]]
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[[Category: Tesar, C.]]
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[[Category: Alkaline phosphatase synthesis sensor protein phor]]
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[[Category: Bacillus subtili]]
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[[Category: Kinase]]
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[[Category: Mcsg]]
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[[Category: Membrane]]
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[[Category: Midwest center for structural genomic]]
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[[Category: Pas domain]]
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[[Category: Phosphate transport]]
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[[Category: Phosphoprotein]]
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[[Category: Protein structure initiative]]
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[[Category: Psi-2]]
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[[Category: Structural genomic]]
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[[Category: Transferase]]
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[[Category: Transmembrane]]
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[[Category: Transport]]
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[[Category: Two-component regulatory system]]
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Current revision

Crystal structure of PAS domain of two-component sensor histidine kinase

PDB ID 3cwf

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