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| | ==Crystal complex of SYS-1/POP-1 at 2.5A resolution== | | ==Crystal complex of SYS-1/POP-1 at 2.5A resolution== |
| - | <StructureSection load='3c2g' size='340' side='right' caption='[[3c2g]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='3c2g' size='340' side='right'caption='[[3c2g]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3c2g]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C2G FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3c2g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C2G FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3c2h|3c2h]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SYS-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans]), POP-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c2g OCA], [https://pdbe.org/3c2g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c2g RCSB], [https://www.ebi.ac.uk/pdbsum/3c2g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c2g ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c2g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c2g RCSB], [http://www.ebi.ac.uk/pdbsum/3c2g PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CTNH1_CAEEL CTNH1_CAEEL] Transcription coregulator (PubMed:26073017). Part of the Wnt signaling asymmetry pathway, probably acting downstream of putative frizzled ligand mom-2, Wnt/frizzled receptors lin-17 and mom-5, and dishevelled homolog dsh-2 (PubMed:15020416, PubMed:17296929, PubMed:17567664, PubMed:19386265, PubMed:26073017). Activates or represses target gene expression, depending on upstream Wnt signals and interactions with transcription factors, such as pop-1 (PubMed:26073017, PubMed:35660370). Required for the activation of Wnt-responsive genes in the E blastomere; thereby leading to a role in endoderm specification and gut development (PubMed:17296929, PubMed:17567664). Reciprocal distribution patterns of sys-1 and pop-1/TCF in the daughters of anterior-posterior cell divisions functions in specifying cell fate; a higher sys-1 to pop-1 ratio promotes the posterior cell fate, whereas a low sys-1 to pop-1 ratio promotes the anterior fate (PubMed:17296929, PubMed:17567664). Represses expression of homeobox ttx-3 in neuroblasts of the SIAD/SIBV lineage, perhaps acting by blocking its transcriptional activation by a complex consisting of ref-2 and pop-1 (PubMed:26073017). Required for early organization of the hermaphrodite, but not the male, gonad; involved in generation of regulatory cells, known as the distal tip cells (DTC), and in formation of the somatic gonadal primordium (PubMed:11161562, PubMed:15020416, PubMed:17296929, PubMed:35196020). Involved in regulating asymmetric divisions of the somatic gonadal precursor cells (SGP), Z1 and Z4 (PubMed:15020416).<ref>PMID:11161562</ref> <ref>PMID:15020416</ref> <ref>PMID:17296929</ref> <ref>PMID:17567664</ref> <ref>PMID:19386265</ref> <ref>PMID:26073017</ref> <ref>PMID:35196020</ref> <ref>PMID:35660370</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 3c2g" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Caenorhabditis elegans]] | | [[Category: Caenorhabditis elegans]] |
| - | [[Category: Amaya, M F.]] | + | [[Category: Large Structures]] |
| - | [[Category: Kimble, J.]] | + | [[Category: Amaya MF]] |
| - | [[Category: Liu, J.]] | + | [[Category: Kimble J]] |
| - | [[Category: Phillips, B T.]] | + | [[Category: Liu J]] |
| - | [[Category: Xu, W.]] | + | [[Category: Phillips BT]] |
| - | [[Category: Beta-catenin]]
| + | [[Category: Xu W]] |
| - | [[Category: Caenorhabditis elegan]]
| + | |
| - | [[Category: Cell adhesion-transcription complex]]
| + | |
| - | [[Category: Developmental protein]]
| + | |
| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: Phylogeny]]
| + | |
| - | [[Category: Pop-1]]
| + | |
| - | [[Category: Sys-1]]
| + | |
| Structural highlights
Function
CTNH1_CAEEL Transcription coregulator (PubMed:26073017). Part of the Wnt signaling asymmetry pathway, probably acting downstream of putative frizzled ligand mom-2, Wnt/frizzled receptors lin-17 and mom-5, and dishevelled homolog dsh-2 (PubMed:15020416, PubMed:17296929, PubMed:17567664, PubMed:19386265, PubMed:26073017). Activates or represses target gene expression, depending on upstream Wnt signals and interactions with transcription factors, such as pop-1 (PubMed:26073017, PubMed:35660370). Required for the activation of Wnt-responsive genes in the E blastomere; thereby leading to a role in endoderm specification and gut development (PubMed:17296929, PubMed:17567664). Reciprocal distribution patterns of sys-1 and pop-1/TCF in the daughters of anterior-posterior cell divisions functions in specifying cell fate; a higher sys-1 to pop-1 ratio promotes the posterior cell fate, whereas a low sys-1 to pop-1 ratio promotes the anterior fate (PubMed:17296929, PubMed:17567664). Represses expression of homeobox ttx-3 in neuroblasts of the SIAD/SIBV lineage, perhaps acting by blocking its transcriptional activation by a complex consisting of ref-2 and pop-1 (PubMed:26073017). Required for early organization of the hermaphrodite, but not the male, gonad; involved in generation of regulatory cells, known as the distal tip cells (DTC), and in formation of the somatic gonadal primordium (PubMed:11161562, PubMed:15020416, PubMed:17296929, PubMed:35196020). Involved in regulating asymmetric divisions of the somatic gonadal precursor cells (SGP), Z1 and Z4 (PubMed:15020416).[1] [2] [3] [4] [5] [6] [7] [8]
Publication Abstract from PubMed
C. elegans SYS-1 has key functional characteristics of a canonical beta-catenin, but no significant sequence similarity. Here, we report the SYS-1 crystal structure, both on its own and in a complex with POP-1, the C. elegans TCF homolog. The two structures possess signature features of canonical beta-catenin and the beta-catenin/TCF complex that could not be predicted by sequence. Most importantly, SYS-1 bears 12 armadillo repeats and the SYS-1/POP-1 interface is anchored by a conserved salt-bridge, the "charged button." We also modeled structures for three other C. elegans beta-catenins to predict the molecular basis of their distinct binding properties. Finally, we generated a phylogenetic tree, using the region of highest structural similarity between SYS-1 and beta-catenin, and found that SYS-1 clusters robustly within the beta-catenin clade. We conclude that the SYS-1 protein belongs to the beta-catenin family and suggest that additional divergent beta-catenins await discovery.
The C. elegans SYS-1 protein is a bona fide beta-catenin.,Liu J, Phillips BT, Amaya MF, Kimble J, Xu W Dev Cell. 2008 May;14(5):751-61. PMID:18477457[9]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Miskowski J, Li Y, Kimble J. The sys-1 gene and sexual dimorphism during gonadogenesis in Caenorhabditis elegans. Dev Biol. 2001 Feb 1;230(1):61-73. PMID:11161562 doi:10.1006/dbio.2000.9998
- ↑ Siegfried KR, Kidd AR 3rd, Chesney MA, Kimble J. The sys-1 and sys-3 genes cooperate with Wnt signaling to establish the proximal-distal axis of the Caenorhabditis elegans gonad. Genetics. 2004 Jan;166(1):171-86. PMID:15020416 doi:10.1534/genetics.166.1.171
- ↑ Phillips BT, Kidd AR 3rd, King R, Hardin J, Kimble J. Reciprocal asymmetry of SYS-1/beta-catenin and POP-1/TCF controls asymmetric divisions in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3231-6. PMID:17296929 doi:10.1073/pnas.0611507104
- ↑ Huang S, Shetty P, Robertson SM, Lin R. Binary cell fate specification during C. elegans embryogenesis driven by reiterated reciprocal asymmetry of TCF POP-1 and its coactivator beta-catenin SYS-1. Development. 2007 Jul;134(14):2685-95. PMID:17567664 doi:10.1242/dev.008268
- ↑ Bertrand V, Hobert O. Linking asymmetric cell division to the terminal differentiation program of postmitotic neurons in C. elegans. Dev Cell. 2009 Apr;16(4):563-75. PMID:19386265 doi:10.1016/j.devcel.2009.02.011
- ↑ Murgan S, Kari W, Rothbächer U, Iché-Torres M, Mélénec P, Hobert O, Bertrand V. Atypical Transcriptional Activation by TCF via a Zic Transcription Factor in C. elegans Neuronal Precursors. Dev Cell. 2015 Jun 22;33(6):737-45. PMID:26073017 doi:10.1016/j.devcel.2015.04.018
- ↑ Thompson JW, Michel MFV, Phillips BT. Centrosomal enrichment and proteasomal degradation of SYS-1/β-catenin requires the microtubule motor dynein. Mol Biol Cell. 2022 May 1;33(5):ar42. PMID:35196020 doi:10.1091/mbc.E22-02-0031
- ↑ Rumley JD, Preston EA, Cook D, Peng FL, Zacharias AL, Wu L, Jileaeva I, Murray JI. pop-1/TCF, ref-2/ZIC and T-box factors regulate the development of anterior cells in the C. elegans embryo. Dev Biol. 2022 Sep;489:34-46. PMID:35660370 doi:10.1016/j.ydbio.2022.05.019
- ↑ Liu J, Phillips BT, Amaya MF, Kimble J, Xu W. The C. elegans SYS-1 protein is a bona fide beta-catenin. Dev Cell. 2008 May;14(5):751-61. PMID:18477457 doi:http://dx.doi.org/S1534-5807(08)00109-3
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