1kh1

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[[Image:1kh1.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of Thermus thermophilus HB8 Argininosuccinate Synthetase==
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|PDB= 1kh1 |SIZE=350|CAPTION= <scene name='initialview01'>1kh1</scene>, resolution 2.3&Aring;
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<StructureSection load='1kh1' size='340' side='right'caption='[[1kh1]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1kh1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KH1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KH1 FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Argininosuccinate_synthase Argininosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.5 6.3.4.5]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE= HB8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kh1 OCA], [https://pdbe.org/1kh1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kh1 RCSB], [https://www.ebi.ac.uk/pdbsum/1kh1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kh1 ProSAT], [https://www.topsan.org/Proteins/RSGI/1kh1 TOPSAN]</span></td></tr>
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</table>
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'''Crystal Structure of Thermus thermophilus HB8 Argininosuccinate Synthetase'''
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== Function ==
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[https://www.uniprot.org/uniprot/ASSY_THET8 ASSY_THET8]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Argininosuccinate synthetase catalyzes the ATP-dependent condensation of a citrulline with an aspartate to give argininosuccinate. The three-dimensional structures of the enzyme from Thermus thermophilus HB8 in its free form, complexed with intact ATP, and complexed with an ATP analogue (adenylyl imidodiphosphate) and substrate analogues (arginine and succinate) have been determined at 2.3-, 2.3-, and 1.95-A resolution, respectively. The structure is essentially the same as that of the Escherichia coli argininosuccinate synthetase. The small domain has the same fold as that of a new family of "N-type" ATP pyrophosphatases with the P-loop specific for the pyrophosphate of ATP. However, the enzyme shows the P-loop specific for the gamma-phosphate of ATP. The structure of the complex form is quite similar to that of the native one, indicating that no conformational change occurs upon the binding of ATP and the substrate analogues. ATP and the substrate analogues are bound to the active site with their reaction sites close to one another and located in a geometrical orientation favorable to the catalytic action. The reaction mechanism so far proposed seems to be consistent with the locations of ATP and the substrate analogues. The reaction may proceed without the large conformational change of the enzyme proposed for the catalytic process.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kh/1kh1_consurf.spt"</scriptWhenChecked>
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1KH1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KH1 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of argininosuccinate synthetase from Thermus thermophilus HB8. Structural basis for the catalytic action., Goto M, Nakajima Y, Hirotsu K, J Biol Chem. 2002 May 3;277(18):15890-6. Epub 2002 Feb 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11844799 11844799]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kh1 ConSurf].
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[[Category: Argininosuccinate synthase]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Hirotsu, k.]]
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[[Category: Goto M]]
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[[Category: Nakajima, Y]]
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[[Category: Hirotsu K]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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[[Category: Nakajima Y]]
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[[Category: goto, m.]]
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[[Category: SO4]]
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[[Category: ligase]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:17:22 2008''
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Current revision

Crystal Structure of Thermus thermophilus HB8 Argininosuccinate Synthetase

PDB ID 1kh1

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