1knc

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[[Image:1knc.gif|left|200px]]
 
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{{Structure
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==Structure of AhpD from Mycobacterium tuberculosis, a novel enzyme with thioredoxin-like activity.==
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|PDB= 1knc |SIZE=350|CAPTION= <scene name='initialview01'>1knc</scene>, resolution 2.00&Aring;
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<StructureSection load='1knc' size='340' side='right'caption='[[1knc]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1knc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KNC FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1knc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1knc OCA], [https://pdbe.org/1knc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1knc RCSB], [https://www.ebi.ac.uk/pdbsum/1knc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1knc ProSAT]</span></td></tr>
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</table>
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'''Structure of AhpD from Mycobacterium tuberculosis, a novel enzyme with thioredoxin-like activity.'''
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== Function ==
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[https://www.uniprot.org/uniprot/AHPD_MYCTU AHPD_MYCTU] Antioxidant protein with alkyl hydroperoxidase activity. Required for the reduction of the AhpC active site cysteine residues and for the regeneration of the AhpC enzyme activity.<ref>PMID:10766746</ref> <ref>PMID:11799204</ref> <ref>PMID:12761216</ref> Together with AhpC, DlaT and Lpd, constitutes an NADH-dependent peroxidase active against hydrogen and alkyl peroxides as well as serving as a peroxynitrite reductase, thus protecting the bacterium against reactive nitrogen intermediates and oxidative stress generated by the host immune system.<ref>PMID:10766746</ref> <ref>PMID:11799204</ref> <ref>PMID:12761216</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Mycobacterium tuberculosis (Mtb) mounts a stubborn defense against oxidative and nitrosative components of the immune response. Dihydrolipoamide dehydrogenase (Lpd) and dihydrolipoamide succinyltransferase (SucB) are components of alpha-ketoacid dehydrogenase complexes that are central to intermediary metabolism. We find that Lpd and SucB support Mtb's antioxidant defense. The peroxiredoxin alkyl hydroperoxide reductase (AhpC) is linked to Lpd and SucB by an adaptor protein, AhpD. The 2.0 angstrom AhpD crystal structure reveals a thioredoxin-like active site that is responsive to lipoamide. We propose that Lpd, SucB (the only lipoyl protein detected in Mtb), AhpD, and AhpC together constitute a nicotinamide adenine dinucleotide (reduced)-dependent peroxidase and peroxynitrite reductase. AhpD thus represents a class of thioredoxin-like molecules that enables an antioxidant defense.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kn/1knc_consurf.spt"</scriptWhenChecked>
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1KNC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNC OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Metabolic enzymes of mycobacteria linked to antioxidant defense by a thioredoxin-like protein., Bryk R, Lima CD, Erdjument-Bromage H, Tempst P, Nathan C, Science. 2002 Feb 8;295(5557):1073-7. Epub 2002 Jan 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11799204 11799204]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1knc ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: Bryk R]]
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[[Category: Bryk, R.]]
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[[Category: Erdjument-Bromage H]]
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[[Category: Erdjument-Bromage, H.]]
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[[Category: Lima CD]]
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[[Category: Lima, C D.]]
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[[Category: Nathan C]]
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[[Category: Nathan, C.]]
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[[Category: Tempst P]]
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[[Category: Tempst, P.]]
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[[Category: SO4]]
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[[Category: ahpd]]
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[[Category: disulfide]]
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[[Category: lpd]]
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[[Category: peroxiredoxin]]
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[[Category: redox]]
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[[Category: thioredoxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:19:41 2008''
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Current revision

Structure of AhpD from Mycobacterium tuberculosis, a novel enzyme with thioredoxin-like activity.

PDB ID 1knc

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