1l1w
From Proteopedia
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| - | [[Image:1l1w.gif|left|200px]] | ||
| - | + | ==NMR structure of a SRP19 binding domain in human SRP RNA== | |
| - | + | <StructureSection load='1l1w' size='340' side='right'caption='[[1l1w]]' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[1l1w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L1W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L1W FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |
| - | | | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l1w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l1w OCA], [https://pdbe.org/1l1w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l1w RCSB], [https://www.ebi.ac.uk/pdbsum/1l1w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l1w ProSAT]</span></td></tr> |
| - | + | </table> | |
| - | + | <div style="background-color:#fffaf0;"> | |
| - | ''' | + | == Publication Abstract from PubMed == |
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Assembly of the human signal recognition particle (SRP) requires SRP19 protein to bind to helices 6 and 8 of SRP RNA. In the present study, structure of a 29-mer RNA composing the SRP19 binding site in helix 6 was determined by NMR spectroscopy. The two A:C mismatches were continuously stacked to each other and formed wobble type A:C base pairs. The GGAG tetraloop in helix 6 was found to adopt a similar conformation to that of GNRA tetraloop, suggesting that these tetraloops are included in an extensive new motif GNRR. Compared with the crystal structure of helix 6 in complex with SRP19 determined previously, the GGAG tetraloop in the complex was found to adopt a similar conformation to the free form, although the loop structure becomes more open upon SRP19 binding. Thus, SRP19 is thought to recognize the overall fold of the GGAG loop. | Assembly of the human signal recognition particle (SRP) requires SRP19 protein to bind to helices 6 and 8 of SRP RNA. In the present study, structure of a 29-mer RNA composing the SRP19 binding site in helix 6 was determined by NMR spectroscopy. The two A:C mismatches were continuously stacked to each other and formed wobble type A:C base pairs. The GGAG tetraloop in helix 6 was found to adopt a similar conformation to that of GNRA tetraloop, suggesting that these tetraloops are included in an extensive new motif GNRR. Compared with the crystal structure of helix 6 in complex with SRP19 determined previously, the GGAG tetraloop in the complex was found to adopt a similar conformation to the free form, although the loop structure becomes more open upon SRP19 binding. Thus, SRP19 is thought to recognize the overall fold of the GGAG loop. | ||
| - | + | Solution structure of a SRP19 binding domain in human SRP RNA.,Sakamoto T, Morita S, Tabata K, Nakamura K, Kawai G J Biochem. 2002 Aug;132(2):177-82. PMID:12153712<ref>PMID:12153712</ref> | |
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| - | Solution structure of a SRP19 binding domain in human SRP RNA., Sakamoto T, Morita S, Tabata K, Nakamura K, Kawai G | + | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 1l1w" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Kawai G]] | ||
| + | [[Category: Morita S]] | ||
| + | [[Category: Nakamura K]] | ||
| + | [[Category: Sakamoto T]] | ||
| + | [[Category: Tabata K]] | ||
Current revision
NMR structure of a SRP19 binding domain in human SRP RNA
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