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1l2f

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[[Image:1l2f.gif|left|200px]]
 
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{{Structure
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==Crystal structure of NusA from Thermotoga maritima: a structure-based role of the N-terminal domain==
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|PDB= 1l2f |SIZE=350|CAPTION= <scene name='initialview01'>1l2f</scene>, resolution 2.50&Aring;
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<StructureSection load='1l2f' size='340' side='right'caption='[[1l2f]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1l2f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L2F FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l2f OCA], [https://pdbe.org/1l2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l2f RCSB], [https://www.ebi.ac.uk/pdbsum/1l2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l2f ProSAT], [https://www.topsan.org/Proteins/BSGC/1l2f TOPSAN]</span></td></tr>
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</table>
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'''Crystal structure of NusA from Thermotoga maritima: a structure-based role of the N-terminal domain'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q9X298_THEMA Q9X298_THEMA]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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We report the crystal structure of N-utilizing substance A protein (NusA) from Thermotoga maritima (TmNusA), a protein involved in transcriptional pausing, termination, and antitermination. TmNusA has an elongated rod-shaped structure consisting of an N-terminal domain (NTD, residues 1-132) and three RNA binding domains (RBD). The NTD consists of two subdomains, the globular head and the helical body domains, that comprise a unique three-dimensional structure that may be important for interacting with RNA polymerase. The globular head domain possesses a high content of negatively charged residues that may interact with the positively charged flaplike domain of RNA polymerase. The helical body domain is composed of a three-helix bundle that forms a hydrophobic core with the aid of two neighboring beta-strands. This domain shows structural similarity with one of the helical domains of sigma(70) factor from Escherichia coli. One side of the molecular surface shows positive electrostatic potential suitable for nonspecific RNA interaction. The RBD is composed of one S1 domain and two K-homology (KH) domains forming an elongated RNA binding surface. Structural comparison between TmNusA and Mycobacterium tuberculosis NusA reveals a possible hinge motion between NTD and RBD. In addition, a functional implication of the NTD in its interaction with RNA polymerase is discussed.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l2/1l2f_consurf.spt"</scriptWhenChecked>
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1L2F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2F OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of NusA from Thermotoga maritima and functional implication of the N-terminal domain., Shin DH, Nguyen HH, Jancarik J, Yokota H, Kim R, Kim SH, Biochemistry. 2003 Nov 25;42(46):13429-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14621988 14621988]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l2f ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
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[[Category: BSGC, Berkeley Structural Genomics Center.]]
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[[Category: Jancarik J]]
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[[Category: Jancarik, J.]]
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[[Category: Kim R]]
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[[Category: Kim, R.]]
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[[Category: Kim SH]]
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[[Category: Kim, S H.]]
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[[Category: Nguyen HH]]
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[[Category: Nguyen, H H.]]
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[[Category: Shin DH]]
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[[Category: Shin, D H.]]
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[[Category: Yokota H]]
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[[Category: Yokota, H.]]
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[[Category: berkeley structural genomics center]]
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[[Category: bsgc structure funded by nih]]
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[[Category: crystal structure]]
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[[Category: nusa]]
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[[Category: ob fold kh domain]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: rna polymerase]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:25:24 2008''
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Current revision

Crystal structure of NusA from Thermotoga maritima: a structure-based role of the N-terminal domain

PDB ID 1l2f

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