1l2k

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Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.

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-[[Image:1l2k.gif|left|200px]]
- {{Structure
+==Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.==
-|PDB= 1l2k |SIZE=350|CAPTION= <scene name='initialview01'>1l2k</scene>, resolution 1.50&Aring;
+<StructureSection load='1l2k' size='340' side='right'caption='[[1l2k]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ND4:AMMONIUM+CATION+WITH+D'>ND4</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=DOD:DEUTERATED WATER'>DOD</scene>
+<table><tr><td colspan='2'>[[1l2k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L2K FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Neutron Diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DOD:DEUTERATED+WATER'>DOD</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ND4:AMMONIUM+CATION+WITH+D'>ND4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l2k OCA], [https://pdbe.org/1l2k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l2k RCSB], [https://www.ebi.ac.uk/pdbsum/1l2k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l2k ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MYG_PHYMC MYG_PHYMC] Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l2/1l2k_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l2k ConSurf].
+<div style="clear:both"></div>
-'''Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.'''
+==See Also==
+*[[Myoglobin 3D structures|Myoglobin 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-From the first days of protein neutron structure determination sperm whale myoglobin was an object under investigation [Nature 224 (1969) 143, J. Mol. Biol. 220 (1991) 381]. Nevertheless myoglobin is still of interest [Proc. Natl. Acad. Sci. USA 97 (2000) 3872]. The feasibility of the monochromatic neutron diffractometer BIX-3 at the JRR-3M reactor at the JAERI [J. Phys. Chem. Solids 60 (1999) 1623], to collect high-resolution diffraction data in a relatively short time stimulated us to repeat the structural determination of myoglobin. The structure of metmyoglobin has been determined up to a resolution of 1.5 A. The hydrogen atoms were replaced in part, by deuterium soaking the crystals for more than 10 years in D(2)O. A refinement of all atoms has been performed including the refinement of individual mean square displacements and occupancies of the exchangeable protons in backbone hydrogen bonds. A method is described to show clear negative scattering densities of the H atoms. Water molecules within the protein and on the molecule surface are shown. The exchangeability of H atoms is correlated with structural distribution and flexibility.
+[[Category: Large Structures]]
-==About this Structure==
-L2K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2K OCA].
-==Reference==
-Hydrogen and deuterium in myoglobin as seen by a neutron structure determination at 1.5 A resolution., Ostermann A, Tanaka I, Engler N, Niimura N, Parak FG, Biophys Chem. 2002 Mar 28;95(3):183-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12062378 12062378]
 [[Category: Physeter catodon]]
-[[Category: Single protein]]
+[[Category: Engler N]]
-[[Category: Engler, N.]]
+[[Category: Niimura N]]
-[[Category: Niimura, N.]]
+[[Category: Ostermann A]]
-[[Category: Ostermann, A.]]
+[[Category: Parak FG]]
-[[Category: Parak, F G.]]
+[[Category: Tanaka I]]
-[[Category: Tanaka, I.]]
-[[Category: DOD]]
-[[Category: HEM]]
-[[Category: ND4]]
-[[Category: SO4]]
-[[Category: heme protein]]
-[[Category: hydration structure]]
-[[Category: hydrogen atom]]
-[[Category: neutron structure]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:25:31 2008''

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1l2k

Current revision

Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.

Structural highlights

Function

Evolutionary Conservation

See Also

Contents

Proteopedia Page Contributors and Editors (what is this?)