1l5a

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:29, 14 February 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1l5a.gif|left|200px]]
 
-
{{Structure
+
==Crystal Structure of VibH, an NRPS Condensation Enzyme==
-
|PDB= 1l5a |SIZE=350|CAPTION= <scene name='initialview01'>1l5a</scene>, resolution 2.55&Aring;
+
<StructureSection load='1l5a' size='340' side='right'caption='[[1l5a]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[1l5a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L5A FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
-
|GENE= vibH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [https://pdbe.org/1l5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB], [https://www.ebi.ac.uk/pdbsum/1l5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l5a ProSAT]</span></td></tr>
-
}}
+
</table>
-
 
+
== Function ==
-
'''Crystal Structure of VibH, an NRPS Condensation Enzyme'''
+
[https://www.uniprot.org/uniprot/Q9KTV9_VIBCH Q9KTV9_VIBCH]
-
 
+
== Evolutionary Conservation ==
-
 
+
[[Image:Consurf_key_small.gif|200px|right]]
-
==Overview==
+
Check<jmol>
-
Nonribosomal peptide synthetases (NRPSs) are large, multidomain enzymes that biosynthesize medically important natural products. We report the crystal structure of the free-standing NRPS condensation (C) domain VibH, which catalyzes amide bond formation in the synthesis of vibriobactin, a Vibrio cholerae siderophore. Despite low sequence identity, NRPS condensation enzymes are structurally related to chloramphenicol acetyltransferase (CAT) and dihydrolipoamide acyltransferases. However, although the latter enzymes are homotrimers, VibH is a monomeric pseudodimer. The VibH structure is representative of both NRPS condensation and epimerization domains, as well as the condensation-variant cyclization domains, which are all expected to be monomers. Surprisingly, despite favorable positioning in the active site, a universally conserved histidine important in CAT and in other C domains is not critical for general base catalysis in VibH.
+
<jmolCheckbox>
-
 
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l5/1l5a_consurf.spt"</scriptWhenChecked>
-
==About this Structure==
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
1L5A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA].
+
<text>to colour the structure by Evolutionary Conservation</text>
-
 
+
</jmolCheckbox>
-
==Reference==
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l5a ConSurf].
-
The structure of VibH represents nonribosomal peptide synthetase condensation, cyclization and epimerization domains., Keating TA, Marshall CG, Walsh CT, Keating AE, Nat Struct Biol. 2002 Jul;9(7):522-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12055621 12055621]
+
<div style="clear:both"></div>
-
[[Category: Single protein]]
+
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
-
[[Category: Keating, A E.]]
+
[[Category: Keating AE]]
-
[[Category: Keating, T A.]]
+
[[Category: Keating TA]]
-
[[Category: Marshall, C G.]]
+
[[Category: Marshall CG]]
-
[[Category: Walsh, C T.]]
+
[[Category: Walsh CT]]
-
[[Category: amide synthase]]
+
-
[[Category: nonribosomal peptide synthetase]]
+
-
[[Category: nrps condensation domain]]
+
-
[[Category: vibriobactin]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:26:33 2008''
+

Current revision

Crystal Structure of VibH, an NRPS Condensation Enzyme

PDB ID 1l5a

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1l5a"
Personal tools