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4wt8
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex== | |
| + | <StructureSection load='4wt8' size='340' side='right'caption='[[4wt8]], [[Resolution|resolution]] 3.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4wt8]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4wus 4wus], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4wwe 4wwe] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4wwt 4wwt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WT8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3V6:BACTOBOLIN+A'>3V6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wt8 OCA], [https://pdbe.org/4wt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wt8 RCSB], [https://www.ebi.ac.uk/pdbsum/4wt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wt8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RS2_THET8 RS2_THET8] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The ribosome is the target of a large number of antibiotics. Here, we report a 3.4-A-resolution crystal structure of bactobolin A bound to 70S ribosome-tRNA complex. The antibiotic binds at a previously unseen site in the 50S subunit and displaces tRNA bound at the P-site. It thus likely has a similar mechanism of action as blasticidin S despite binding to a different site. The structure also rationalizes previously identified resistance mutations. | ||
| - | + | Bactobolin A Binds to a Site on the 70S Ribosome Distinct from Previously Seen Antibiotics.,Amunts A, Fiedorczuk K, Truong TT, Chandler J, Peter Greenberg E, Ramakrishnan V J Mol Biol. 2015 Jan 3. pii: S0022-2836(14)00654-8. doi:, 10.1016/j.jmb.2014.12.018. PMID:25562208<ref>PMID:25562208</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4wt8" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Thermus thermophilus HB8]] | ||
| + | [[Category: Amunts A]] | ||
| + | [[Category: Fiedorczuk K]] | ||
| + | [[Category: Ramakrishnan V]] | ||
Current revision
Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex
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