4wwj
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==UndA, an oxygen-activating, non-heme iron dependent desaturase/decarboxylase== | |
| + | <StructureSection load='4wwj' size='340' side='right'caption='[[4wwj]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4wwj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_protegens_Pf-5 Pseudomonas protegens Pf-5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WWJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwj OCA], [https://pdbe.org/4wwj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wwj RCSB], [https://www.ebi.ac.uk/pdbsum/4wwj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wwj ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q4K8M0_PSEF5 Q4K8M0_PSEF5] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Aliphatic medium-chain 1-alkenes (MCAEs, approximately 10 carbons) are "drop-in" compatible next-generation fuels and precursors to commodity chemicals. Mass production of MCAEs from renewable resources holds promise for mitigating dependence on fossil hydrocarbons. An MCAE, such as 1-undecene, is naturally produced by Pseudomonas as a semivolatile metabolite through an unknown biosynthetic pathway. We describe here the discovery of a single gene conserved in Pseudomonas responsible for 1-undecene biosynthesis. The encoded enzyme is able to convert medium-chain fatty acids (C10-C14) into their corresponding terminal olefins using an oxygen-activating, nonheme iron-dependent mechanism. Both biochemical and X-ray crystal structural analyses suggest an unusual mechanism of beta-hydrogen abstraction during fatty acid substrate activation. Our discovery unveils previously unidentified chemistry in the nonheme Fe(II) enzyme family, provides an opportunity to explore the biology of 1-undecene in Pseudomonas, and paves the way for tailored bioconversion of renewable raw materials to MCAE-based biofuels and chemical commodities. | ||
| - | + | Microbial biosynthesis of medium-chain 1-alkenes by a nonheme iron oxidase.,Rui Z, Li X, Zhu X, Liu J, Domigan B, Barr I, Cate JH, Zhang W Proc Natl Acad Sci U S A. 2014 Dec 8. pii: 201419701. PMID:25489112<ref>PMID:25489112</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4wwj" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas protegens Pf-5]] | ||
| + | [[Category: Cate JDH]] | ||
| + | [[Category: Li X]] | ||
Current revision
UndA, an oxygen-activating, non-heme iron dependent desaturase/decarboxylase
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