1lf6

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[[Image:1lf6.gif|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE==
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|PDB= 1lf6 |SIZE=350|CAPTION= <scene name='initialview01'>1lf6</scene>, resolution 2.10&Aring;
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<StructureSection load='1lf6' size='340' side='right'caption='[[1lf6]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1lf6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LF6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LF6 FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Glucan_1,4-alpha-glucosidase Glucan 1,4-alpha-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.3 3.2.1.3]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lf6 OCA], [https://pdbe.org/1lf6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lf6 RCSB], [https://www.ebi.ac.uk/pdbsum/1lf6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lf6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O85672_THETR O85672_THETR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lf/1lf6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lf6 ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE'''
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==See Also==
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*[[Alpha-glucosidase 3D structures|Alpha-glucosidase 3D structures]]
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*[[Amylase 3D structures|Amylase 3D structures]]
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==Overview==
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__TOC__
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The first crystal structures of a two-domain, prokaryotic glucoamylase were determined to high resolution from the clostridial species Thermoanaerobacterium thermosaccharolyticum with and without acarbose. The N-terminal domain has 18 antiparallel strands arranged in beta-sheets of a super-beta-sandwich. The C-terminal domain is an (alpha/alpha)(6) barrel, lacking the peripheral subdomain of eukaryotic glucoamylases. Interdomain contacts are common to all prokaryotic Family GH15 proteins. Domains similar to those of prokaryotic glucoamylases in maltose phosphorylases (Family GH65) and glycoaminoglycan lyases (Family PL8) suggest evolution from a common ancestor. Eukaryotic glucoamylases may have evolved from prokaryotic glucoamylases by the substitution of the N-terminal domain with the peripheral subdomain and by the addition of a starch-binding domain.
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</StructureSection>
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[[Category: Large Structures]]
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==About this Structure==
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1LF6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LF6 OCA].
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==Reference==
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Crystal structure and evolution of a prokaryotic glucoamylase., Aleshin AE, Feng PH, Honzatko RB, Reilly PJ, J Mol Biol. 2003 Mar 14;327(1):61-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12614608 12614608]
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[[Category: Glucan 1,4-alpha-glucosidase]]
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[[Category: Single protein]]
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[[Category: Thermoanaerobacterium thermosaccharolyticum]]
[[Category: Thermoanaerobacterium thermosaccharolyticum]]
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[[Category: Aleshin, A E.]]
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[[Category: Aleshin AE]]
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[[Category: Feng, P H.]]
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[[Category: Feng P-H]]
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[[Category: Honzatko, R B.]]
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[[Category: Honzatko RB]]
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[[Category: Reilly, P J.]]
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[[Category: Reilly PJ]]
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[[Category: SO4]]
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[[Category: (alpha/alpha) barrel]]
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[[Category: 6 alpha-helical hairpin torroid]]
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[[Category: carbohydrase family gh15]]
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[[Category: super beta sandwich]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:30:13 2008''
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Current revision

CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE

PDB ID 1lf6

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